糖尿病和非糖尿病血清中白蛋白与果糖胺浓度的关系。

Relationship between albumin and fructosamine concentration in diabetic and non-diabetic sera.

作者信息

Johnson R N, Metcalf P A, Baker J R

出版信息

Clin Chim Acta. 1987 Apr 30;164(2):151-62. doi: 10.1016/0009-8981(87)90066-0.

Abstract

We investigated the contribution of albumin to serum fructosamine activity. On gel exclusion chromatography, pooled diabetic and pooled non-diabetic sera before and after incubation with [14C]glucose differed in fructosamine activity and radioactivity mainly in the albumin-containing fractions. We confirmed the major contribution of glycated albumin to serum fructosamine concentration using affinity chromatography on Blue Sepharose. The importance of high molecular mass species was further demonstrated by the recovery of reducing activity following dialysis and charcoal treatment. Fructosamine measurements in diabetic patients were not correlated with serum albumin concentration in individuals with urinary albumin less than 1 g/l. We conclude that fructosamine activity is a convenient measure of glycosylprotein concentration and that the index responds mainly to glycation of albumin. However, routine correction for serum albumin concentration is inappropriate.

摘要

我们研究了白蛋白对血清果糖胺活性的作用。在凝胶排阻色谱上，与[¹⁴C]葡萄糖孵育前后的糖尿病患者混合血清和非糖尿病患者混合血清，其果糖胺活性和放射性主要在含白蛋白的组分中存在差异。我们使用蓝色琼脂糖亲和色谱法证实了糖化白蛋白对血清果糖胺浓度的主要贡献。透析和活性炭处理后还原活性的恢复进一步证明了高分子量物质的重要性。在尿白蛋白低于1 g/l的个体中，糖尿病患者的果糖胺测量值与血清白蛋白浓度无关。我们得出结论，果糖胺活性是糖基化蛋白浓度的一种便捷测量方法，且该指标主要反映白蛋白的糖化情况。然而，对血清白蛋白浓度进行常规校正并不合适。