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骨髓间充质干细胞（BMMSCs）与脉冲电磁场（PEMF）联合应用对促进小鼠脊髓损伤后恢复的增强作用。

Enhanced effect of combining bone marrow mesenchymal stem cells (BMMSCs) and pulsed electromagnetic fields (PEMF) to promote recovery after spinal cord injury in mice.

作者信息

Huang Liyi, Sun Xin, Wang Lu, Pei Gaiqing, Wang Yang, Zhang Qing, Liang Zejun, Wang Dong, Fu Chenying, He Chengqi, Wei Quan

机构信息

Rehabilitation Medicine Center and Institute of Rehabilitation Medicine, West China Hospital Sichuan University Chengdu PR China.

Key Laboratory of Rehabilitation Medicine in Sichuan Province Sichuan University Chengdu PR China.

出版信息

MedComm (2020). 2022 Aug 3;3(3):e160. doi: 10.1002/mco2.160. eCollection 2022 Sep.

DOI:10.1002/mco2.160
PMID:35949547
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9350428/
Abstract

Spinal cord injury (SCI) is a destructive traumatic disease of the central nervous system without satisfying therapy efficiency. Bone marrow mesenchymal stem cells (BMMSCs) therapy promotes the neurotrophic factors' secretion and axonal regeneration, thereby promoting recovery of SCI. Pulsed electromagnetic fields (PEMF) therapy has been proven to promote neural growth and regeneration. Both BMMSCs and PEMF have shown curative effects for SCI; PEMF can further promote stem cell differentiation. Thus, we explored the combined effects of BMMSCs and PEMF and the potential interaction between these two therapies in SCI. Compared with the SCI control, BMMSCs, and PEMF groups, the combinational therapy displayed the best therapeutic effect. Combinational therapy increased the expression levels of nutritional factors including brain-derived neurotrophic factor (BDNF), nerve growth factors (NGF) and vascular endothelial growth factor (VEGF), enhanced neuron preservation (NeuN and NF-200), and increased axonal growth (MBP and myelin sheath). Additionally, PEMF promoted the expression levels of BDNF and VEGF in BMMSCs via Wnt/β-catenin signaling pathway. In summary, the combined therapy of BMMSCs and PEMF displayed a more satisfactory effect than BMMSCs and PEMF therapy alone, indicating a promising application of combined therapy for the therapy of SCI.

摘要

脊髓损伤（SCI）是一种中枢神经系统的破坏性创伤性疾病，目前尚无令人满意的治疗效果。骨髓间充质干细胞（BMMSCs）疗法可促进神经营养因子的分泌和轴突再生，从而促进脊髓损伤的恢复。脉冲电磁场（PEMF）疗法已被证明可促进神经生长和再生。BMMSCs和PEMF对脊髓损伤均显示出治疗效果；PEMF可进一步促进干细胞分化。因此，我们探讨了BMMSCs和PEMF的联合作用以及这两种疗法在脊髓损伤中的潜在相互作用。与脊髓损伤对照组、BMMSCs组和PEMF组相比，联合治疗显示出最佳的治疗效果。联合治疗提高了包括脑源性神经营养因子（BDNF）、神经生长因子（NGF）和血管内皮生长因子（VEGF）在内的营养因子的表达水平，增强了神经元保护（NeuN和NF-200），并增加了轴突生长（MBP和髓鞘）。此外，PEMF通过Wnt/β-连环蛋白信号通路促进BMMSCs中BDNF和VEGF的表达水平。总之，BMMSCs和PEMF联合治疗比单独使用BMMSCs和PEMF治疗显示出更令人满意的效果，表明联合治疗在脊髓损伤治疗中有广阔的应用前景。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/594f/9350428/e66ca1ee8fbc/MCO2-3-e160-g003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/594f/9350428/c2731d8ee51c/MCO2-3-e160-g007.jpg
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