Dejnek Maciej, Witkowski Jarosław, Moreira Helena, Płaczkowska Sylwia, Morasiewicz Piotr, Reichert Paweł, Królikowska Aleksandra
Department of Trauma Surgery, Wroclaw Medical University, Wroclaw 50-556, Poland.
Department of Medical Science Foundation, Wroclaw Medical University, Wroclaw 50-556, Poland.
World J Orthop. 2022 Jun 18;13(6):587-602. doi: 10.5312/wjo.v13.i6.587.
The evaluation of the efficacy of platelet-rich plasma (PRP) in clinical practice yields conflicting results and raises numerous controversies. This may be due to different concentrations of biologically active components in PRP obtained with the use of different methods of gravity separation.
To compare the content, repeatability and correlations between biologically active components in PRP obtained with four different commercial systems.
From a whole blood sample of each of 12 healthy male volunteers, 4 PRP samples were prepared using 4 different commercial kits [Arthrex Autologous Conditioned Plasma (ACP), Mini GPS III, Xerthra, Dr. PRP] in accordance with the instructions provided by the manufacturers. A comparative analysis of blood cell components - 13 selected inflammatory cytokines and 7 growth factors - in the obtained PRP samples was performed using the Kruskal-Wallis test by ranks. The repeatability of results in each method was evaluated by the estimation of the coefficient of variation. The Spearman correlation was used to estimate the relationship between blood cell content and cytokines.
Significantly higher concentrations of platelets (PLT), white blood cells (WBC) and red blood cells (RBC) were found in PRP obtained with the use of Mini GPS III than in PRP obtained using other systems. Significant differences in the content of growth factors and cytokines in PRP were found. A positive correlation of the amount of PLT, RBC and WBC with the concentration of most of the growth factors was found but in only three inflammatory cytokines. The obtained correlations between blood cell components and cytokines differed between the systems in terms of statistical significance, which may be due to insufficient sample size. The repeatability of the obtained PLT concentration also varied between protocols with the lowest in Xerthra and the highest in Arthrex ACP.
Significant differences in the content of biologically active components and their repeatability were found in PRP obtained by various methods, providing new data for further research.
在临床实践中，对富血小板血浆（PRP）疗效的评估结果相互矛盾，并引发了众多争议。这可能是由于使用不同重力分离方法获得的PRP中生物活性成分浓度不同所致。
比较用四种不同商业系统获得的PRP中生物活性成分的含量、重复性及相关性。
根据制造商提供的说明，使用4种不同的商业试剂盒[Arthrex自体条件血浆（ACP）、Mini GPS III、Xerthra、Dr. PRP]，从12名健康男性志愿者每人的全血样本中制备4份PRP样本。使用Kruskal-Wallis秩和检验对所获PRP样本中的血细胞成分、13种选定的炎性细胞因子和7种生长因子进行比较分析。通过估计变异系数评估每种方法结果的重复性。采用Spearman相关性分析评估血细胞含量与细胞因子之间的关系。
发现使用Mini GPS III获得的PRP中血小板（PLT）、白细胞（WBC）和红细胞（RBC）的浓度显著高于使用其他系统获得的PRP。PRP中生长因子和细胞因子的含量存在显著差异。发现PLT、RBC和WBC的数量与大多数生长因子的浓度呈正相关，但仅与三种炎性细胞因子呈正相关。血细胞成分与细胞因子之间获得的相关性在不同系统之间的统计学意义有所不同，这可能是由于样本量不足所致。所获PLT浓度的重复性在不同方案之间也有所不同，Xerthra中最低，Arthrex ACP中最高。
不同方法获得的PRP中生物活性成分的含量及其重复性存在显著差异，为进一步研究提供了新数据。
