和的毒性研究及抑菌作用
Toxicity Study and Antibacterial Effects of the Leaves Extracts of and .
机构信息
Department of Microbial, Cellular and Molecular Biology, College of Natural and Computational Sciences, Addis Ababa University, Ethiopia.
Department of Biology, College of Natural and Computational Sciences, Arba Minch University, Ethiopia.
出版信息
Ethiop J Health Sci. 2022 Jul;32(4):823-832. doi: 10.4314/ejhs.v32i4.20.
BACKGROUND
The objective of this study was to evaluate the in vivo toxicity and antibacterial activity of the leaves extracts of Boscia coriacea and Uvaria leptocladon.
METHODS
Extraction was performed using 80% methanol by maceration and Soxhlet extraction method. Evaluation of the acute toxicity of the extracts was based on the Organization for Economic Cooperation and Development (OECD) guideline. Evaluation of antibacterial activity of the extracts was done by agar well diffusion assay. Determinations of minimum inhibitory concentrations (MIC) of the extracts were performed by broth macro-dilution method. The checkerboard method was used for the determination of combined effect of antibiotics and the extracts. Paired T-test and one way analysis of variance were used for statistical analysis.
RESULTS
B. coriacea and U. leptocladon have no toxic effect in Swiss albino mice up to dose of 5000 mg/kg. B. coriacea and U. leptocladon showed antibacterial activity at concentration of 500 mg/ml. The chloroform-methanol fraction of B. coriacea and U. leptocladon showed the highest antibacterial activity at concentration of 25 mg/ml. The MIC and minimum bactericidal concentration (MBC) of B. coriacea were 125 mg/ml and 250 mg/ml, respectively. The MIC of U. leptocladon ranged from 31.25 mg/ml to 62.5 mg/ml, while its MBC ranged from 62.5 to 125 mg/ml. The combination assay of B. coriacea and the antibiotics showed additive effect, while U. leptocladon and the antibiotics showed indifferent effect.
CONCLUSION
The findings showed that U. leptocladon and B. coriacea leaves extracts have antibacterial activity and no toxicity in animal model.
背景
本研究旨在评估扁担柴和鹰爪花叶片提取物的体内毒性和抗菌活性。
方法
采用 80%甲醇索氏提取法和浸渍法提取。根据经济合作与发展组织（OECD）指南评估提取物的急性毒性。采用琼脂打孔扩散法评价提取物的抗菌活性。采用肉汤微量稀释法测定提取物的最低抑菌浓度（MIC）。采用棋盘法测定抗生素与提取物联合作用的协同效应。采用配对 T 检验和单因素方差分析进行统计学分析。
结果
在 5000mg/kg 剂量下，扁担柴和鹰爪花对瑞士白化小鼠没有毒性作用。扁担柴和鹰爪花在 500mg/ml 浓度下显示出抗菌活性。扁担柴和鹰爪花的氯仿-甲醇部分在 25mg/ml 浓度下表现出最高的抗菌活性。扁担柴的 MIC 和最低杀菌浓度（MBC）分别为 125mg/ml 和 250mg/ml。鹰爪花的 MIC 范围为 31.25mg/ml 至 62.5mg/ml，MBC 范围为 62.5mg/ml 至 125mg/ml。扁担柴与抗生素联合检测显示相加作用，而鹰爪花与抗生素联合检测显示无关作用。
结论
研究结果表明，扁担柴和鹰爪花叶片提取物在动物模型中具有抗菌活性且无毒性。
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