School of Stomatology, Dalian Medical University, Dalian, 116044, Liaoning, China.
Department of Basic Science of Stomatology, Shanghai Stomatological Hospital, Fudan University, Shanghai, 200003, China.
J Nanobiotechnology. 2022 Aug 11;20(1):370. doi: 10.1186/s12951-022-01580-w.
Small extracellular vesicles (sEVs) are an important component in the paracrine pathway. They can be used as a substitute for seed cells and have shown good application prospects in promoting bone regeneration. Cow's milk could be used as a source of sEVs with good biocompatibility and cost-effectiveness, with easy availability, low cost and low toxicity. This study focused on the role and mechanism of small extracellular vesicles derived from milk in bone repair. In order to explore the mechanism via which Milk-sEVs promote bone repair, we screened the differential gene GJA1 in Milk-sEV-treated osteoblasts through transcriptome chips, and verified the transcript AP3B1 of GJA1 through chromatin immunoprecipitation (CHIP). We have proved by in vivo and in vitro experiments that milk-derived sEVs (Milk-sEVs) increase the repair ability of bone tissue, and promote expression of the osteogenic gene GJA1 through the transcript AP3B1.
小细胞外囊泡 (sEVs) 是旁分泌途径中的重要组成部分。它们可以用作种子细胞的替代品，在促进骨再生方面具有良好的应用前景。牛的牛奶可以作为 sEVs 的来源，具有良好的生物相容性和成本效益，且易于获得、成本低、毒性低。本研究专注于牛奶来源的 sEVs 在骨修复中的作用和机制。为了探索 Milk-sEVs 促进骨修复的机制，我们通过转录组芯片筛选出 Milk-sEV 处理的成骨细胞中的差异基因 GJA1，并通过染色质免疫沉淀 (CHIP) 验证了 GJA1 的转录本 AP3B1。通过体内和体外实验证明，牛奶来源的 sEV（Milk-sEVs）增加了骨组织的修复能力，并通过转录本 AP3B1 促进了成骨基因 GJA1 的表达。
