Di Shujuan, Huang Yibo, Qiao Weicang, Zhang Xiaomei, Wang Yaling, Zhang Minghui, Fu Jieyu, Zhao Junying, Chen Lijun
Key Laboratory of Dairy Science, Ministry of Education, Food Science College, Northeast Agricultural University, Harbin, China.
National Engineering Research Center of Dairy Health for Maternal and Child, Beijing Sanyuan Foods Co. Ltd., Beijing, China.
Front Nutr. 2024 Dec 16;11:1512939. doi: 10.3389/fnut.2024.1512939. eCollection 2024.
Milk-derived extracellular vesicles (EVs) have various functions, including immune regulation and promoting intestinal development. These EVs have substantial potential for application in infant formula and functional foods development. In addition, numerous studies have shown that milk-derived EVs carry proteins, lipids, and nucleic acids away from their parental cells, acting as messengers between cells. Moreover, structural integrity and biological viability are necessary prerequisites for the functional and omics studies of milk-derived EVs. Therefore, selecting appropriate methods for isolating and characterizing milk-derived EVs is essential for subsequent studies. Accordingly, this review summarizes the isolation and characterization methods for milk-derived EVs and their biological functions and roles. Furthermore, it discusses the comprehensive application of isolation methods, providing a reference for research on and development of milk-derived EVs.
乳源细胞外囊泡（EVs）具有多种功能，包括免疫调节和促进肠道发育。这些细胞外囊泡在婴儿配方奶粉和功能性食品开发中具有巨大的应用潜力。此外，大量研究表明，乳源细胞外囊泡携带着蛋白质、脂质和核酸离开其亲本细胞，充当细胞间的信使。此外，结构完整性和生物活性是乳源细胞外囊泡功能和组学研究的必要前提条件。因此，选择合适的方法分离和表征乳源细胞外囊泡对于后续研究至关重要。据此，本综述总结了乳源细胞外囊泡的分离和表征方法及其生物学功能和作用。此外，还讨论了分离方法的综合应用，为乳源细胞外囊泡的研发提供参考。
