Huang Shuotian, Zhang Lixia, Cai Tingting, Zhao Yuxuan, Liu Jiao, Wu Pengfei, Ma Xiangqing, Shuai Peng
College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Chinese Fir Engineering Technology Research Center of the State Forestry and Grassland Administration, Fuzhou 350002, China.
Plants (Basel). 2022 Aug 4;11(15):2036. doi: 10.3390/plants11152036.
Chinese fir ( (Lamb.) Hook) is a widely grown gymnosperm in China. Phosphorus (P) is an indispensable nutrient for the growth of Chinese fir. Inorganic phosphate (Pi) deficiency exists in soils of many Chinese fir planting area regions, and the trees themselves have limited efficiency in utilizing P from the soil. Ethylene is important in regulation responses to nutrient deficiencies. However, little is known about how ethylene signals participate in Pi stress in Chinese fir. A total of six different treatments were performed to reveal the transcript levels of Chinese fir under Pi, ethephon (an ethylene-releasing compound), and CoCl (cobalt chloride, an ethylene biosynthesis inhibitor) treatments. We assembled a full-length reference transcriptome containing 22,243 unigenes as a reference for UMI RNA-seq (Digital RNA-seq). There were 586 Differentially Expressed Genes (DEGs) in the Pi starvation (NP) group, while DEGs from additional ethephon or CoCl in NP were 708 and 292, respectively. Among the DEGs in each treatment, there were 83 TFs in these treatment groups. MYB (v-myb avian myeloblastosis viral oncogene homolog) family was the most abundant transcription factors (TFs). Three ERF (Ethylene response factor) family genes were identified when only ethylene content was imposed as a variable. Enrichment analysis indicated that the ascorbate and aldarate metabolism pathway plays a key role in resistance to Pi deficiency. This study provides insights for further elucidating the regulatory mechanism of Pi deficiency in Chinese fir.
杉木（(Lamb.) Hook）是中国广泛种植的裸子植物。磷（P）是杉木生长不可或缺的养分。许多杉木种植区的土壤中存在无机磷（Pi）缺乏的情况，而且树木自身从土壤中吸收磷的效率有限。乙烯在对养分缺乏的调节反应中很重要。然而，关于乙烯信号如何参与杉木对Pi胁迫的反应却知之甚少。总共进行了六种不同的处理，以揭示杉木在Pi、乙烯利（一种释放乙烯的化合物）和CoCl（氯化钴，一种乙烯生物合成抑制剂）处理下的转录水平。我们组装了一个包含22,243个单基因的全长参考转录组，作为UMI RNA测序（数字RNA测序）的参考。Pi饥饿（NP）组中有586个差异表达基因（DEG），而NP组中额外添加乙烯利或CoCl后的DEG分别为708个和292个。在每种处理的DEG中，这些处理组中有83个转录因子（TF）。MYB（v-myb禽成髓细胞瘤病毒癌基因同源物）家族是最丰富的转录因子（TF）。当仅将乙烯含量作为变量时，鉴定出三个乙烯反应因子（ERF）家族基因。富集分析表明，抗坏血酸和醛糖代谢途径在抵抗Pi缺乏中起关键作用。本研究为进一步阐明杉木Pi缺乏的调控机制提供了见解。
