Makar Lara E, Nady Norhan, Abd El-Fattah Ahmed, Shawky Neivin, Kandil Sherif H
Department of Materials Science, Institute of Graduate Studies and Research, Alexandria University, El-Shatby, Alexandria 21526, Egypt.
Polymeric Materials Research Department, City of Scientific Research and Technological Applications (SRTA-City), Alexandria 21934, Egypt.
Polymers (Basel). 2022 Jul 28;14(15):3052. doi: 10.3390/polym14153052.
In this work, physical cross-linking was used to create nanocomposite hydrogels composed of unmodified gum arabic (GA), chitosan (Ch), and natural nanohydroxyapatite (nHA), using an acrylic acid (AA) solvent. Different GA/chitosan contents (15%, 25%, and 35% of the used AA) as well as different nHA contents (2, 5, and 10 wt.%), were used and studied. The natural nHA and the fabricated GA/Ch/nHA nanocomposite hydrogels were characterized using different analysis techniques. Using acrylic acid solvent produced novel hydrogels with compressive strength of 15.43-22.20 MPa which is similar to that of natural cortical bone. The addition of natural nHA to the hydrogels resulted in a significant improvement in the compressive strength of the fabricated hydrogels. In vitro studies of water absorption and degradation-and in vivo studies-confirmed that the nanocomposite hydrogels described here are biodegradable, biocompatible, and facilitate apatite formation while immersed in the simulated body fluid (SBF). In light of these findings, the GA/Ch/nHA nanocomposite hydrogels are recommended for preparing bioactive nanoscaffolds for testing in bone regeneration applications.
在这项工作中，采用物理交联法，以丙烯酸（AA）为溶剂，制备了由未改性阿拉伯胶（GA）、壳聚糖（Ch）和天然纳米羟基磷灰石（nHA）组成的纳米复合水凝胶。使用了不同的GA/壳聚糖含量（占所用AA的15%、25%和35%）以及不同的nHA含量（2、5和10 wt.%）并进行了研究。采用不同的分析技术对天然nHA和制备的GA/Ch/nHA纳米复合水凝胶进行了表征。使用丙烯酸溶剂制备出了抗压强度为15.43 - 22.20 MPa的新型水凝胶，这与天然皮质骨的抗压强度相似。向水凝胶中添加天然nHA可显著提高制备的水凝胶的抗压强度。吸水性和降解的体外研究以及体内研究证实，本文所述的纳米复合水凝胶具有生物可降解性、生物相容性，并且在浸入模拟体液（SBF）时有助于磷灰石形成。鉴于这些发现，推荐使用GA/Ch/nHA纳米复合水凝胶制备生物活性纳米支架，用于骨再生应用测试。
