Department of Pharmacology, Faculty of Medicine, Srinakharinwirot University, Bangkok 10110, Thailand.
Expert Center of Innovative Agriculture, Thailand Institute of Scientific and Technological Research (TISTR), Pathum Thani 12120, Thailand.
Molecules. 2022 Aug 8;27(15):5034. doi: 10.3390/molecules27155034.
The main cause of most skin cancers is damage from UVB from sunlight, which penetrate the skin surface and induce inflammation. For this reason, this study aims to identify natural products with photo-protection properties and their mode of action by using the UVB-irradiated HaCaT keratinocyte model. fruit extracts at 25, 50, and 100 µg/mL recovered cell viability following UVB exposure in a dose-dependent manner. Cell survival was associated with the reduction in intracellular ROS and NO. In addition, we showed that the pre-treatment with the fruit extract lowered the phosphorylation level of two MAPK-signaling pathways: p38 MAPKs and JNKs. The resulting lower MAPK activation decreased their downstream pro-inflammatory cascade through COX-2 expression and subsequently reduced the PGE proinflammatory mediator level. The photoprotective effects of the fruit extract were correlated with the presence of polyphenolic compounds, including cyanidin, ferulic acid, caffeic acid, vanillic acid, and protocatechuic acid, which have been previously described as antioxidant and anti-inflammation. Together, we demonstrated that the pre-treatment with the fruit extract had photo-protection by inhibiting oxidative stress and subsequently lowered stress-induced MAPK responses. Therefore, this fresh fruit is worthy of investigation to be utilized as a skincare ingredient for preventing UVB-induced skin damage.
大多数皮肤癌的主要成因是来自阳光的 UVB 所造成的损害，这些损害会穿透皮肤表面并引发炎症。基于此，本研究旨在通过使用经 UVB 照射的 HaCaT 角质形成细胞模型，来识别具有光保护特性及其作用模式的天然产物。研究发现，25、50 和 100μg/mL 的果实提取物在剂量依赖性的方式下，可恢复经 UVB 照射后的细胞活力。细胞存活率与细胞内 ROS 和 NO 的减少有关。此外，我们表明，果实提取物的预处理降低了两条 MAPK 信号通路：p38 MAPKs 和 JNKs 的磷酸化水平。较低的 MAPK 激活降低了其下游的促炎级联反应，通过 COX-2 表达，并随后降低 PGE 促炎介质的水平。果实提取物的光保护作用与多酚类化合物的存在相关，包括先前被描述为抗氧化和抗炎作用的矢车菊素、阿魏酸、咖啡酸、香草酸和原儿茶酸。总之，我们证明了果实提取物的预处理具有光保护作用，可抑制氧化应激，并随后降低应激诱导的 MAPK 反应。因此，这种新鲜水果值得进一步研究，以作为预防 UVB 诱导的皮肤损伤的护肤品成分。
