Ganilho Catarina, da Silva Márcia Bessa, Paiva Cristiana, de Menezes Thacilla Ingrid, Dos Santos Mayara Roncaglia, Pereira Carlos M, Pereira Ruth, Andreani Tatiana
GreenUPorto, Sustainable Agrifood Production Research Centre & INOV4AGRO, Department of Biology, Faculty of Sciences, University of Porto, Rua Campo Alegre s/n, 4169-007 Porto, Portugal.
Chemistry Research Centre (CIQUP) & Institute of Molecular Sciences (IMS), Department of Chemistry and Biochemistry, Faculty of Sciences, University of Porto, Rua do Campo Alegre s/n, 4169-007 Porto, Portugal.
Nanomaterials (Basel). 2022 Jul 27;12(15):2576. doi: 10.3390/nano12152576.
Lipid nanoparticles (LN) composed of biodegradable lipids and produced by green methods are candidates for the encapsulation of pesticides, potentially contributing to decreasing their release in the environment. From a safety-by-design concept, this work proposes LN for the encapsulation of insecticide active ingredients (AI). However, given the complexity of nanoparticles, ecotoxicological studies are often controversial, and a detailed investigation of their effects on the environment is required. Accordingly, this work aimed to produce and characterize LN containing the insecticide lambda-cyhalothrin (LC) and evaluate their safety to crops ( and ), soil invertebrates ( and ), and soil microbial parameters. The average particle size for LN-loaded with LC (LN-LC) was 165.4 ± 2.34 nm, with narrow size distribution and negative charge (-38.7 ± 0.954 mV). LN were able to encapsulate LC with an entrapment efficacy of 98.44 ± 0.04%, maintaining the stability for at least 4 months. The LN-LC showed no risk to the growth of crops and reproduction of the invertebrates. The effect on microbial parameters showed that the activity of certain soil microbial parameters can be inhibited or stimulated by the presence of LN at highest concentrations, probably by changing the pH of soil or by the intrinsic properties of LN.
由可生物降解脂质组成并通过绿色方法制备的脂质纳米颗粒（LN）是用于封装农药的候选材料，可能有助于减少其在环境中的释放。从设计安全的概念出发，这项工作提出将LN用于封装杀虫剂活性成分（AI）。然而，鉴于纳米颗粒的复杂性，生态毒理学研究往往存在争议，因此需要对其对环境的影响进行详细调查。因此，这项工作旨在制备并表征含有杀虫剂高效氯氟氰菊酯（LC）的LN，并评估其对作物（和）、土壤无脊椎动物（和）以及土壤微生物参数的安全性。负载LC的LN（LN-LC）的平均粒径为165.4±2.34nm，粒径分布窄且带负电荷（-38.7±0.954mV）。LN能够以98.44±0.04%的包封率封装LC，并保持至少4个月的稳定性。LN-LC对作物生长和无脊椎动物繁殖没有风险。对微生物参数的影响表明，在最高浓度下，LN的存在可能会通过改变土壤pH值或LN的固有特性来抑制或刺激某些土壤微生物参数的活性。
