Liang Zhonghou, Li Xiang, Li Ping, Deng Ye, Zhong Yuan, Yang Hua
Hunan Engineering Research Center for Applied Technology of Understory Medicinal Plants, Hengyang 421005, China.
Hunan Polytechnic of Environment and Biology, Hengyang 421005, China.
J Oncol. 2022 Aug 1;2022:3152155. doi: 10.1155/2022/3152155. eCollection 2022.
is an important resource of traditional Chinese medicine. We find out the gene information of enzymes related to lignan biosynthesis and metabolism of , so as to provide a scientific basis for the breeding of new varieties of . In this paper, 2-year-old from Hunan provincial germplasm resource bank was used as the material and its root, stem, and leaf were analyzed by extensive targeted metabolomics combined with transcriptome sequencing. The results showed the following: (1) 51 lignans were detected by metabolome analysis, and the content of lignans in roots was higher than that in stems and leaves. The high content of lignans in roots, stems, and leaves includes ring-opening isolarch phenol-4-o-glucoside, narrow leaf schisandrin E, and schisandrin B. (2) After transcriptome sequencing, 13 classes of 137 Unigenes related to lignan biosynthesis pathway were retrieved. The analysis of differential genes in different parts showed that the overall expression amount and species of lignan synthase gene in stems and leaves were closer than those in roots. CCoAOMT, C3H, and SIDR gene families are mainly expressed in roots and stems. (3) Metabolome combined with transcriptome analysis further screened these genes and obtained 11 genes of enzyme gene families such as HCT, DIR, COMT, CAD, SIDR, and PLR, which are highly correlated in lignan synthesis. Therefore, there are many lignans and their synthase-related genes in roots, stems, and leaves, but the content and expression of different lignans and their synthase-related genes are quite different in each part. In this study, the gene information of the lignan biosynthesis enzyme was obtained for the first time, which laid a good foundation for the cloning and molecular breeding of the key enzyme gene of lignan biosynthesis.
是重要的中药材资源。我们找出了与木脂素生物合成和代谢相关的酶的基因信息，以便为新品种的培育提供科学依据。本文以湖南省种质资源库两年生的为材料，采用广泛靶向代谢组学结合转录组测序技术对其根、茎、叶进行分析。结果如下：（1）代谢组分析检测到51种木脂素，根中木脂素含量高于茎和叶。根、茎、叶中木脂素含量较高的有开环异落叶松脂酚-4-o-葡萄糖苷、狭叶五味子酯E和五味子醇甲。（2）转录组测序后，检索到137个与木脂素生物合成途径相关的13类单基因。不同部位差异基因分析表明，茎和叶中木脂素合酶基因的整体表达量和种类比根中更接近。CCoAOMT、C3H和SIDR基因家族主要在根和茎中表达。（3）代谢组结合转录组分析进一步筛选这些基因，获得了HCT、DIR、COMT、CAD、SIDR和PLR等酶基因家族中11个在木脂素合成中高度相关的基因。因此，根、茎、叶中存在多种木脂素及其合酶相关基因，但不同木脂素及其合酶相关基因在各部位的含量和表达差异较大。本研究首次获得了木脂素生物合成酶的基因信息，为木脂素生物合成关键酶基因的克隆和分子育种奠定了良好基础。
