Seemakram Wasan, Paluka Jakkapat, Suebrasri Thanapat, Lapjit Chanon, Kanokmedhakul Somdej, Kuyper Thomas W, Ekprasert Jindarat, Boonlue Sophon
Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand.
Natural Products Research Unit, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand.
Front Plant Sci. 2022 Jul 26;13:845794. doi: 10.3389/fpls.2022.845794. eCollection 2022.
This study aimed to investigate the efficiency of arbuscular mycorrhizal fungi (AMF) to promote growth and cannabinoid yield of KKU05. A completely randomized design (CRD) was conducted with six replications for 60 days. Two different species of AMF, PC2-2 and BM-3 g3 were selected as inocula and compared with two non-mycorrhizal controls, one without synthetic fertilizer and one with synthetic NPK fertilizer. The unfertilized non-mycorrhizal plants had the lowest performance, whereas plants inoculated with BM-3 g3 performed best, both in terms of plant biomass and concentrations of CBD and THC. There were no significant differences in plant biomass and cannabinoid concentrations between non-mycorrhizal plants that received synthetic fertilizer and mycorrhizal plants with inoculum of PC2-2. Our data demonstrate the great potential for cannabis cultivation without risking deterioration of soil structure, such as soil hardening and increased acidity, which might be induced by long-term use of synthetic fertilizer.
本研究旨在调查丛枝菌根真菌（AMF）促进KKU05生长和大麻素产量的效率。采用完全随机设计（CRD），重复6次，为期60天。选择两种不同的AMF物种PC2-2和BM-3 g3作为接种物，并与两个非菌根对照进行比较，一个不施合成肥料，另一个施合成氮磷钾肥料。未施肥的非菌根植物表现最差，而接种BM-3 g3的植物在植物生物量以及CBD和THC浓度方面表现最佳。接受合成肥料的非菌根植物与接种PC2-2的菌根植物在植物生物量和大麻素浓度方面没有显著差异。我们的数据表明，大麻种植具有巨大潜力，不会有土壤结构恶化的风险，如长期使用合成肥料可能导致的土壤硬化和酸度增加。
