Kuchel P W, Chapman B E, Potts J R
FEBS Lett. 1987 Jul 13;219(1):5-10. doi: 10.1016/0014-5793(87)81180-8.
We present the results of a new NMR-based procedure for measuring the fast transmembrane exchange of D-[1-13C]glucose in human erythrocytes. The method relies on different rates of exchange between the alpha- and beta-anomers of glucose inside and outside the cells; the rate outside the cells is greatly increased by the addition of mutarotase to the suspension. Theory is developed to describe nuclear-spin transfer in the present system and is used to analyse the data to yield estimates of transmembrane-exchange rate constants and their statistical uncertainties. For a total glucose concentration of 25.5 mmol/l at 40 degrees C the first order efflux rate constants for the alpha- and beta-anomers were 1.20 +/- 0.40 s-1 and 0.71 +/- 0.30 s-1, respectively.
我们展示了一种基于核磁共振的新方法用于测量人红细胞中D-[1-13C]葡萄糖的快速跨膜交换结果。该方法依赖于细胞内外葡萄糖α-和β-异头物之间不同的交换速率；向悬浮液中添加变旋酶可大大提高细胞外的速率。我们建立了理论来描述当前系统中的核自旋转移，并用于分析数据以得出跨膜交换速率常数及其统计不确定性的估计值。在40℃下总葡萄糖浓度为25.5 mmol/l时，α-和β-异头物的一级流出速率常数分别为1.20±0.40 s-1和0.71±0.30 s-1。
