Kuchel P W, Bulliman B T, Chapman B E
Department of Biochemistry, University of Sydney, NSW, Australia.
Biophys Chem. 1988 Oct;32(1):89-95. doi: 10.1016/0301-4622(88)85037-3.
The rates of exchange between the alpha- and beta-anomers of D-[1-13C]glucose, at equilibrium catalyzed by porcine kidney mutarotase (EC 5.1.3.3), were measured using 13C-NMR spin-transfer procedures. This entailed inversion-transfer and saturation-transfer experiments, and two-dimensional exchange spectroscopy (2D EXSY). The concentration and temperature dependences of the fluxes were studied; equilibrium exchange Michaelis constants, and the activation energy of the catalyzed reaction were thereby measured.
使用¹³C-NMR自旋转移方法测量了猪肾变旋酶（EC 5.1.3.3）催化下D-[1-¹³C]葡萄糖的α-和β-异头物在平衡时的交换速率。这需要进行反转转移和饱和转移实验以及二维交换光谱（2D EXSY）。研究了通量的浓度和温度依赖性；从而测量了平衡交换米氏常数以及催化反应的活化能。
