攻击一个“已死”的问题：DEAD -box ATPases 在核糖体组装及其他方面的作用。
Attacking a DEAD problem: The role of DEAD-box ATPases in ribosome assembly and beyond.
机构信息
Department of Integrative Structural and Computational Biology, Scripps Florida, Jupiter, FL, United States; HHMI Faculty Scholar, Chevy Chase, MD, United States; The Skaggs Graduate School of Chemical and Biological Sciences, Scripps Florida, Jupiter, FL, United States.
出版信息
Methods Enzymol. 2022;673:19-38. doi: 10.1016/bs.mie.2022.03.033. Epub 2022 Apr 9.
Abstract
DEAD-box proteins are a subfamily of ATPases with similarity to RecA-type helicases that are involved in all aspects of RNA Biology. Despite their potential to regulate these processes via their RNA-dependent ATPase activity, their roles remain poorly characterized. Here I describe a roadmap to study these proteins in the context of ribosome assembly, the process that utilizes more than half of all DEAD-box proteins encoded in the yeast genome.
摘要
DEAD-box 蛋白是一类与 RecA 型解旋酶具有相似性的 ATP 酶亚家族，它们参与 RNA 生物学的各个方面。尽管它们可以通过其 RNA 依赖的 ATP 酶活性来调节这些过程，但它们的作用仍然知之甚少。在这里，我描述了一条研究这些蛋白在核糖体组装背景下的路线图，核糖体组装是一个利用酵母基因组中编码的一半以上 DEAD-box 蛋白的过程。
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