光驱动单细胞旋转粘附频率测定法。
Light-driven single-cell rotational adhesion frequency assay.
作者信息
Liu Yaoran, Ding Hongru, Li Jingang, Lou Xin, Yang Mingcheng, Zheng Yuebing
机构信息
Department of Electrical and Computer Engineering, The University of Texas at Austin, Austin, TX 78712 USA.
Walker Department of Mechanical Engineering, The University of Texas at Austin, Austin, TX 78712 USA.
出版信息
eLight. 2022;2(1):13. doi: 10.1186/s43593-022-00020-4. Epub 2022 Aug 8.
UNLABELLED
The interaction between cell surface receptors and extracellular ligands is highly related to many physiological processes in living systems. Many techniques have been developed to measure the ligand-receptor binding kinetics at the single-cell level. However, few techniques can measure the physiologically relevant shear binding affinity over a single cell in the clinical environment. Here, we develop a new optical technique, termed single-cell rotational adhesion frequency assay (scRAFA), that mimics in vivo cell adhesion to achieve label-free determination of both homogeneous and heterogeneous binding kinetics of targeted cells at the subcellular level. Moreover, the scRAFA is also applicable to analyze the binding affinities on a single cell in native human biofluids. With its superior performance and general applicability, scRAFA is expected to find applications in study of the spatial organization of cell surface receptors and diagnosis of infectious diseases.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1186/s43593-022-00020-4.
未标注
细胞表面受体与细胞外配体之间的相互作用与生命系统中的许多生理过程高度相关。已经开发出许多技术来在单细胞水平上测量配体 - 受体结合动力学。然而，在临床环境中，很少有技术能够测量单个细胞上与生理相关的剪切结合亲和力。在此，我们开发了一种新的光学技术，称为单细胞旋转粘附频率测定法（scRAFA），它模拟体内细胞粘附，以在亚细胞水平上实现对靶向细胞的均相和异质结合动力学的无标记测定。此外，scRAFA还适用于分析天然人类生物流体中单个细胞上的结合亲和力。凭借其卓越的性能和广泛的适用性，scRAFA有望在细胞表面受体的空间组织研究和传染病诊断中找到应用。
补充信息
在线版本包含可在10.1186/s43593 - 022 - 00020 - 4获取的补充材料。
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