The synergistic effect of and on triple-negative breast cancer.

BACKGROUND

Accumulating evidence suggests that long non-coding ribonucleic acid (RNA) cyclin-dependent kinase inhibitor 2B antisense RNA 1 () and messenger RNA (mRNA) spindle component 25 () contribute to tumorigenesis and progression in various cancers. However, the synergistic effect between and has not yet been fully elucidated in triple-negative breast cancer (TNBC). This study sought to examine the synergistic effect of and and uncover a novel mechanism for the progression of TNBC.

METHODS

The transcriptome profiles of TNBC in The Cancer Genome Atlas (TCGA) were calculated for differentially expressed genes (DEGs). Gene co-expression networks were constructed via a weighted correlation network analysis. We validated the relationship between and by bioinformatics and studies (including Cell Counting Kit-8, transwell assays, and quantitative real-time polymerase chain reaction).

RESULTS

was found to be carcinogenic and was significantly upregulated and co-expressed with elevated expression levels in the TNBC cells and sequencing profiles. Notably, the mRNA levels were associated with poor clinical outcomes in TNBC patients. Specifically, the knockdown of significantly inhibited TNBC cell proliferation and migration.

CONCLUSIONS

We identified a novel cancer-promoting regulation axis. The co-expression of and is expected to serve as a powerful candidate biomarker for diagnostic and prognostic purposes in TNBC.