Liu Pan, Wang Lili, Xing Sining, Zhao Song, Yu Huiying
Laboratory of Basic Medicine, General Hospital of Northern Theater Command, Shenyang, 110016, Liaoning, China.
Beifang Hospital of China Medical University, Shenyang, China.
Sci Rep. 2025 Jul 21;15(1):26414. doi: 10.1038/s41598-025-09615-6.
In the development of human cancers, alterations in the process of cell mitosis frequently play a role. In recent years, an increasing number of researchers have focused on the phenomenon of cancer resulting from kinetochore-microtubule attachment imbalance.Spindle pole body component 25 (SPC25) is essential for the organization of the spindle devices and chromosome separation. SPC25 has a significant role in the occurrence and development of malignant tumors, but its role in lung adenocarcinoma has not been established. This study utilizes the TCGA database and centrosome assembly checkpoint specialized resources to explore the molecular mechanisms of lung adenocarcinoma and its interaction with the immune system. By using the "limma" and "survival" R software packages and Venn diagram analysis, we identify differentially expressed mRNAs that are associated with lung adenocarcinoma prognosis and play a role in centrosome assembly checkpoint regulation, and determine SPC25 as the target gene. We use the "pROC" software package to draw ROC curves to evaluate its diagnostic potential, perform chi-square tests and logistic regression models to analyze its relationship with clinical pathological features. Cox regression analysis deepens our understanding of its prognostic evaluation role, and enrichment analysis reveals its biological functions and signaling pathways. We also explore the correlation between SPC25 and lung adenocarcinoma immune infiltration by combining ssGSEA and Spearman analysis. The objective of this study was to investigate the association between SPC25 and the prognosis as well as immune infiltration in lung adenocarcinoma, aiming to establish a crucial molecular foundation for early non-invasive diagnosis and immunotherapy of lung adenocarcinoma.
在人类癌症的发展过程中，细胞有丝分裂过程的改变常常发挥作用。近年来，越来越多的研究人员关注到因动粒-微管附着失衡导致癌症的现象。纺锤极体组件25（SPC25）对于纺锤体装置的组织和染色体分离至关重要。SPC25在恶性肿瘤的发生和发展中具有重要作用，但其在肺腺癌中的作用尚未明确。本研究利用TCGA数据库和中心体组装检查点专门资源，探讨肺腺癌的分子机制及其与免疫系统的相互作用。通过使用“limma”和“survival”R软件包以及维恩图分析，我们鉴定出与肺腺癌预后相关且在中心体组装检查点调节中起作用的差异表达mRNA，并确定SPC25为靶基因。我们使用“pROC”软件包绘制ROC曲线以评估其诊断潜力，进行卡方检验和逻辑回归模型分析其与临床病理特征的关系。Cox回归分析加深了我们对其预后评估作用的理解，富集分析揭示了其生物学功能和信号通路。我们还通过结合单样本基因集富集分析（ssGSEA）和Spearman分析，探讨SPC25与肺腺癌免疫浸润之间的相关性。本研究的目的是探讨SPC25与肺腺癌预后及免疫浸润之间的关联，旨在为肺腺癌的早期非侵入性诊断和免疫治疗建立关键的分子基础。
