Yamazaki Kyoko, Miyauchi Eiji, Kato Tamotsu, Sato Keisuke, Suda Wataru, Tsuzuno Takahiro, Yamada-Hara Miki, Sasaki Nobuo, Ohno Hiroshi, Yamazaki Kazuhisa
Division of Periodontology, Niigata University Graduate School of Medical and Dental Sciences, Niigata Japan.
Laboratory for Intestinal Ecosystem, RIKEN Centre for Integrative Medical Sciences (IMS), Kanagawa Japan.
J Oral Microbiol. 2022 Aug 11;14(1):2110194. doi: 10.1080/20002297.2022.2110194. eCollection 2022.
The effect of oral microbiota on the intestinal microbiota has garnered growing attention as a mechanism linking periodontal diseases to systemic diseases. However, the salivary microbiota is diverse and comprises numerous bacteria with a largely similar composition in healthy individuals and periodontitis patients.
We explored how health-associated and periodontitis-associated salivary microbiota differently colonized the intestine and their subsequent systemic effects.
The salivary microbiota was collected from a healthy individual and a periodontitis patient and gavaged into C57BL/6NJcl[GF] mice. Gut microbial communities, hepatic gene expression profiles, and serum metabolites were analyzed.
The gut microbial composition was significantly different between periodontitis-associated microbiota-administered (PAO) and health-associated oral microbiota-administered (HAO) mice. The hepatic gene expression profile demonstrated a distinct pattern between the two groups, with higher expression of lipid and glucose metabolism-related genes. Disease-associated metabolites such as 2-hydroxyisobutyric acid and hydroxybenzoic acid were elevated in PAO mice. These metabolites were significantly correlated with characteristic gut microbial taxa in PAO mice. Conversely, health-associated oral microbiota were associated with higher levels of beneficial serum metabolites in HAO mice.
The multi-omics approach used in this study revealed that periodontitis-associated oral microbiota is associated with the induction of disease phenotype when they colonized the gut of germ-free mice.
作为将牙周疾病与全身性疾病联系起来的一种机制，口腔微生物群对肠道微生物群的影响已受到越来越多的关注。然而，唾液微生物群具有多样性，在健康个体和牙周炎患者中，其包含的众多细菌组成在很大程度上相似。
我们探讨了与健康相关和与牙周炎相关的唾液微生物群如何以不同方式定殖于肠道及其随后产生的全身影响。
从一名健康个体和一名牙周炎患者收集唾液微生物群，并灌胃给C57BL/6NJcl[GF]小鼠。分析肠道微生物群落、肝脏基因表达谱和血清代谢产物。
给予与牙周炎相关微生物群（PAO）的小鼠和给予与健康相关口腔微生物群（HAO）的小鼠之间，肠道微生物组成存在显著差异。肝脏基因表达谱在两组之间呈现出不同模式，脂质和葡萄糖代谢相关基因的表达更高。PAO小鼠中疾病相关代谢产物如2-羟基异丁酸和羟基苯甲酸升高。这些代谢产物与PAO小鼠中特征性肠道微生物分类群显著相关。相反，在HAO小鼠中，与健康相关的口腔微生物群与有益血清代谢产物的较高水平相关。
本研究中使用的多组学方法表明，与牙周炎相关的口腔微生物群在定殖于无菌小鼠肠道时与疾病表型的诱导有关。
