• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

分析胃癌患者外周血 T 细胞受体库。

Profiling the peripheral blood T cell receptor repertoires of gastric cancer patients.

机构信息

Department of Computer Science, City University of Hong Kong Shenzhen Research Institute, Shenzhen, China.

BGI-Shenzhen, Shenzhen, China.

出版信息

Front Immunol. 2022 Jul 28;13:848113. doi: 10.3389/fimmu.2022.848113. eCollection 2022.

DOI:10.3389/fimmu.2022.848113
PMID:35967453
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9367216/
Abstract

Cancer driven by somatic mutations may express neoantigens that can trigger T-cell immune responses. Since T-cell receptor (TCR) repertoires play critical roles in anti-tumor immune responses for oncology, next-generation sequencing (NGS) was used to profile the hypervariable complementarity-determining region 3 (CDR3) of the TCR-beta chain in peripheral blood samples from 68 gastric cancer patients and 49 healthy controls. We found that most hyper-expanded CDR3 are individual-specific, and the gene usage of TRBV3-1 is more frequent in the tumor group regardless of tumor stage than in the healthy control group. We identified 374 hyper-expanded tumor-specific CDR3, which may play a vital role in anti-tumor immune responses. The patients with stage IV gastric cancer have higher EBV-specific CDR3 abundance than the control. In conclusion, analysis of the peripheral blood TCR repertoires may provide the biomarker for gastric cancer prognosis and guide future immunotherapy.

摘要

由体细胞突变驱动的癌症可能表达新抗原，这些抗原可以触发 T 细胞免疫反应。由于 T 细胞受体 (TCR) 库在肿瘤学的抗肿瘤免疫反应中起着关键作用，因此使用下一代测序 (NGS) 来分析 68 名胃癌患者和 49 名健康对照者外周血样本中 TCR-β 链的高变互补决定区 3 (CDR3)。我们发现，大多数超扩展的 CDR3 是个体特异性的，并且无论肿瘤分期如何，TRBV3-1 的基因使用在肿瘤组中比在健康对照组中更为频繁。我们鉴定了 374 个超扩展的肿瘤特异性 CDR3，它们可能在抗肿瘤免疫反应中发挥重要作用。IV 期胃癌患者的 EBV 特异性 CDR3 丰度高于对照组。总之，外周血 TCR 库分析可能为胃癌预后提供生物标志物，并指导未来的免疫治疗。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e225/9367216/164ba1e09ee4/fimmu-13-848113-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e225/9367216/d10e5b7920ef/fimmu-13-848113-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e225/9367216/5375cf1204d6/fimmu-13-848113-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e225/9367216/7ae99c4069f5/fimmu-13-848113-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e225/9367216/164ba1e09ee4/fimmu-13-848113-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e225/9367216/d10e5b7920ef/fimmu-13-848113-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e225/9367216/5375cf1204d6/fimmu-13-848113-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e225/9367216/7ae99c4069f5/fimmu-13-848113-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e225/9367216/164ba1e09ee4/fimmu-13-848113-g004.jpg

相似文献

1
Profiling the peripheral blood T cell receptor repertoires of gastric cancer patients.分析胃癌患者外周血 T 细胞受体库。
Front Immunol. 2022 Jul 28;13:848113. doi: 10.3389/fimmu.2022.848113. eCollection 2022.
2
High-throughput sequencing of CD4 T cell repertoire reveals disease-specific signatures in IgG4-related disease.CD4 T 细胞 repertoire 的高通量测序揭示了 IgG4 相关疾病中的疾病特异性特征。
Arthritis Res Ther. 2019 Dec 19;21(1):295. doi: 10.1186/s13075-019-2069-6.
3
[T cell repertoires correlate with pathogenesis of chronic idiopathic thrombocytopenic purpura].[T细胞受体库与慢性特发性血小板减少性紫癜的发病机制相关]
Zhonghua Yi Xue Za Zhi. 2005 Dec 14;85(47):3316-22.
4
Characteristics and prognostic significance of profiling the peripheral blood T-cell receptor repertoire in patients with advanced lung cancer.晚期肺癌患者外周血 T 细胞受体谱特征及预后意义。
Int J Cancer. 2019 Sep 1;145(5):1423-1431. doi: 10.1002/ijc.32145. Epub 2019 Jan 31.
5
Analysis of the Repertoire Features of TCR Beta Chain CDR3 in Human by High-Throughput Sequencing.高通量测序分析人类TCRβ链CDR3的 repertoire 特征
Cell Physiol Biochem. 2016;39(2):651-67. doi: 10.1159/000445656. Epub 2016 Jul 21.
6
Diversity of the T cell receptor β chain complementarity-determining region 3 in peripheral blood of neonates with sepsis: an analysis based on immune repertoire sequencing.基于免疫受体测序的新生儿脓毒症外周血 T 细胞受体 β 链互补决定区 3 多样性分析。
Zhongguo Dang Dai Er Ke Za Zhi. 2021 Nov 15;23(11):1154-1160. doi: 10.7499/j.issn.1008-8830.2106044.
7
Next-generation sequencing analysis of the human T-cell and B-cell receptor repertoire diversity before and after hepatitis B vaccination.乙肝疫苗接种前后人类 T 细胞和 B 细胞受体库多样性的下一代测序分析。
Hum Vaccin Immunother. 2019;15(11):2738-2753. doi: 10.1080/21645515.2019.1600987. Epub 2019 Jul 25.
8
Characterization of the T-cell receptor beta chain repertoire in tumor-infiltrating lymphocytes.肿瘤浸润淋巴细胞中T细胞受体β链库的特征分析
Cancer Med. 2016 Sep;5(9):2513-21. doi: 10.1002/cam4.828. Epub 2016 Jul 27.
9
Analysis of the Heterogeneity of CD4CD25 T Cell TCR CDR3 Repertoires in Breast Tumor Tissues, Lung Metastatic Tissues, and Spleens from 4T1 Tumor-Bearing BALB/c Mice.分析 4T1 荷瘤 BALB/c 小鼠乳腺肿瘤组织、肺转移组织和脾脏中 CD4CD25 T 细胞 TCR CDR3 多样性。
J Immunol Res. 2020 Sep 24;2020:3184190. doi: 10.1155/2020/3184190. eCollection 2020.
10
A pilot study to assess peripheral blood TCR β-chain CDR3 repertoire in occupational medicamentosa-like dermatitis due to trichloroethylene using high-throughput sequencing.一项使用高通量测序评估职业性三氯乙烯药疹样皮炎患者外周血 TCR β 链 CDR3 多样性的初步研究。
Environ Toxicol Pharmacol. 2019 Oct;71:103211. doi: 10.1016/j.etap.2019.103211. Epub 2019 Jun 12.

引用本文的文献

1
Integration of T cell repertoire, CyTOF, genotyping and symptomatology data reveals subphenotypic variability in COVID-19 patients.T细胞受体库、质谱流式细胞术、基因分型和症状学数据的整合揭示了COVID-19患者的亚表型变异性。
Comput Struct Biotechnol J. 2025 May 14;27:2063-2073. doi: 10.1016/j.csbj.2025.05.016. eCollection 2025.
2
Interpretable deep learning reveals the role of an E-box motif in suppressing somatic hypermutation of AGCT motifs within human immunoglobulin variable regions.可解释的深度学习揭示了 E 盒基序在抑制人类免疫球蛋白可变区中 AGCT 基序的体细胞高频突变中的作用。
Front Immunol. 2024 May 28;15:1407470. doi: 10.3389/fimmu.2024.1407470. eCollection 2024.
3

本文引用的文献

1
High-throughput and single-cell T cell receptor sequencing technologies.高通量和单细胞 T 细胞受体测序技术。
Nat Methods. 2021 Aug;18(8):881-892. doi: 10.1038/s41592-021-01201-8. Epub 2021 Jul 19.
2
Developing an Unbiased Multiplex PCR System to Enrich the Repertoire Toward Accurate Detection in Leukemia.开发一种无偏倚多重 PCR 系统，以丰富白血病准确检测的 repertoire。
Front Immunol. 2020 Aug 6;11:1631. doi: 10.3389/fimmu.2020.01631. eCollection 2020.
3
Immune-awakening revealed by peripheral T cell dynamics after one cycle of immunotherapy.
Exploration of signature based on T cell-related genes in stomach adenocarcinoma by analysis of single cell sequencing data.
通过单细胞测序数据分析探索基于T细胞相关基因的胃腺癌特征
Aging (Albany NY). 2024 Mar 25;16(7):6035-6053. doi: 10.18632/aging.205687.
4
Advances and key focus areas in gastric cancer immunotherapy: A comprehensive scientometric and clinical trial review (1999-2023).胃癌免疫治疗的进展和重点领域：一项全面的科学计量学和临床试验综述（1999-2023）。
World J Gastroenterol. 2023 Oct 28;29(40):5593-5617. doi: 10.3748/wjg.v29.i40.5593.
免疫疗法一个周期后外周 T 细胞动力学揭示的免疫唤醒。
Nat Cancer. 2020 Feb;1(2):210-221. doi: 10.1038/s43018-019-0022-x. Epub 2020 Feb 10.
4
The Diagnostic, Prognostic, and Therapeutic Potential of Adaptive Immune Receptor Repertoire Profiling in Cancer.癌症中适应性免疫受体谱系分析的诊断、预后和治疗潜力。
Cancer Res. 2020 Feb 15;80(4):643-654. doi: 10.1158/0008-5472.CAN-19-1457. Epub 2019 Dec 30.
5
Investigation of Antigen-Specific T-Cell Receptor Clusters in Human Cancers.探究人类癌症中的抗原特异性 T 细胞受体簇。
Clin Cancer Res. 2020 Mar 15;26(6):1359-1371. doi: 10.1158/1078-0432.CCR-19-3249. Epub 2019 Dec 12.
6
Genetically engineered T cells for cancer immunotherapy.基因工程 T 细胞用于癌症免疫疗法。
Signal Transduct Target Ther. 2019 Sep 20;4:35. doi: 10.1038/s41392-019-0070-9. eCollection 2019.
7
Characterization of Distinct T Cell Receptor Repertoires in Tumor and Distant Non-tumor Tissues from Lung Cancer Patients.从肺癌患者的肿瘤和远处非肿瘤组织中鉴定出独特的 T 细胞受体库。
Genomics Proteomics Bioinformatics. 2019 Jun;17(3):287-296. doi: 10.1016/j.gpb.2018.10.005. Epub 2019 Aug 31.
8
PIRD: Pan Immune Repertoire Database.PIRD：全免疫受体数据库。
Bioinformatics. 2020 Feb 1;36(3):897-903. doi: 10.1093/bioinformatics/btz614.
9
Recent advances in gastric cancer early diagnosis.胃癌早期诊断的最新进展。
World J Gastroenterol. 2019 May 7;25(17):2029-2044. doi: 10.3748/wjg.v25.i17.2029.
10
Using T Cell Receptor Repertoires to Understand the Principles of Adaptive Immune Recognition.利用 T 细胞受体库来理解适应性免疫识别的原理。
Annu Rev Immunol. 2019 Apr 26;37:547-570. doi: 10.1146/annurev-immunol-042718-041757. Epub 2019 Jan 30.