Pham Van T, Greppi Anna, Chassard Christophe, Braegger Christian, Lacroix Christophe
Laboratory of Food Biotechnology, Institute of Food, Nutrition and Health, ETH Zurich, Zurich, Switzerland.
Division of Gastroenterology and Nutrition, University Children's Hospital Zurich, Zurich, Switzerland.
Front Nutr. 2022 Jul 28;9:948131. doi: 10.3389/fnut.2022.948131. eCollection 2022.
The early intestinal colonization of functional microbial groups plays an essential role in infant gut health, with most studies targeting the initial colonization period from birth to 6 months of age. In a previous report, we demonstrated the metabolic cross-feeding of lactate and identified keystone species specified for lactate utilization in fecal samples of 40 healthy infants. We present here the extension of our longitudinal study for the period from 6 months to 2 years, with a focus on the colonization of functional groups involved in lactate metabolism and butyrate production. We captured the dynamic changes of the gut microbiota and reported a switch in the predominant lactate-producing and lactate-utilizing bacteria, from producing propionate in the first year to producing butyrate in the second year of life. The significant increase in butyrate producers and fecal butyrate concentration was also pinpointed to the weaning period between 6 and 10 months. Correlation analyses further suggested, for the first time, the metabolic cross-feeding of hydrogen in infants. In conclusion, our longitudinal study of 40 Swiss infants provides important insights into the colonization of functional groups involved in lactate metabolism and butyrate production in the first 2 years of life.
功能性微生物群的早期肠道定殖对婴儿肠道健康起着至关重要的作用，大多数研究针对的是从出生到6个月大的初始定殖期。在之前的一份报告中，我们展示了乳酸的代谢交叉喂养，并在40名健康婴儿的粪便样本中鉴定出了专门用于乳酸利用的关键物种。我们在此展示了我们从6个月到2岁期间纵向研究的扩展，重点关注参与乳酸代谢和丁酸盐产生的功能组的定殖情况。我们捕捉了肠道微生物群的动态变化，并报告了主要的乳酸产生菌和乳酸利用菌的转变，从生命的第一年产生丙酸盐转变为第二年产生丁酸盐。丁酸盐产生菌和粪便丁酸盐浓度的显著增加也被确定在6至10个月的断奶期。相关性分析首次进一步表明了婴儿体内氢气的代谢交叉喂养。总之，我们对40名瑞士婴儿的纵向研究为生命最初2年中参与乳酸代谢和丁酸盐产生的功能组的定殖提供了重要见解。
