Natural Products Laboratory, International Joint Laboratory of Tea Chemistry and Health Effects, State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, People's Republic of China.
Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Food Funct. 2022 Sep 22;13(18):9299-9310. doi: 10.1039/d2fo01795d.
Green tea polyphenols show positive effects on human health and longevity. However, knowledge of the antiaging properties of green tea is limited to the major catechin epigallocatechin gallate (EGCG). The search for new ingredients in tea with strong antiaging activity deserves further study. Here we isolated and identified two new catechins from Zijuan green tea, named zijuanin E (1) and zijuanin F (2). Their structures were identified by extensive high-resolution mass spectroscopy (HR-MS), nuclear magnetic resonance (NMR), ultraviolet-vis (UV), infrared (IR) and circular dichroism (CD) spectroscopic analyses, and their C NMR and CD data were calculated. We used the nematode () to analyze the health benefits and longevity effects of 1 and 2. Compounds 1 and 2 (100 μM) remarkably prolonged the lifespan of by 67.2% and 56.0%, respectively, delaying the age-related decline of phenotypes, enhancing stress resistance, and reducing ROS and lipid accumulation. Furthermore, 1 and 2 did not affect the lifespan of , , , and mutant worms, suggesting that they might work the insulin/IGF and SKN-1/Nrf2 signaling pathways. Meanwhile, 1 and 2 also exhibited strong antioxidant activity . Surface plasmon resonance (SPR) evidence suggests that zijuanins E and F have strong human serum albumin (HSA) binding ability. Together, zijuanins E and F represent a new valuable class of tea components that promote healthspan and could be developed as potential dietary therapies against aging.
绿茶多酚对人类健康和长寿有积极影响。然而，人们对绿茶抗衰老特性的认识仅限于主要儿茶素表没食子儿茶素没食子酸酯（EGCG）。因此，值得进一步研究寻找具有较强抗衰老活性的茶中新型成分。在这里，我们从紫娟绿茶中分离并鉴定了两种新的儿茶素，分别命名为紫娟因 E（1）和紫娟因 F（2）。通过广泛的高分辨率质谱（HR-MS）、核磁共振（NMR）、紫外可见（UV）、红外（IR）和圆二色（CD）光谱分析，确定了它们的结构，并计算了它们的 13C NMR 和 CD 数据。我们利用线虫（）分析了 1 和 2 的健康益处和长寿效应。化合物 1 和 2（100 μM）分别显著延长了线虫的寿命 67.2%和 56.0%，延缓了与年龄相关的表型下降，增强了应激抗性，并减少了 ROS 和脂质积累。此外，1 和 2 不影响 、 、 和 突变体线虫的寿命，这表明它们可能作用于胰岛素/IGF 和 SKN-1/Nrf2 信号通路。同时，1 和 2 还表现出较强的抗氧化活性。表面等离子体共振（SPR）证据表明，紫娟因 E 和 F 具有很强的人血清白蛋白（HSA）结合能力。总之，紫娟因 E 和 F 代表了一类新的有价值的茶成分，可促进健康寿命的延长，可作为潜在的对抗衰老的饮食疗法进行开发。
