School of Laboratory Medicine, Hubei University of Chinese Medicine, 16 Huangjia Lake West Road, Wuhan, 430065, China.
School of Laboratory Medicine, Hubei University of Chinese Medicine, 16 Huangjia Lake West Road, Wuhan, 430065, China.
Talanta. 2023 Jan 15;252:123764. doi: 10.1016/j.talanta.2022.123764. Epub 2022 Aug 2.
The external acid environment of cancer cells is different from that of normal cells, making a profound impact on cancer progression. Here we report a simple poly-l-lysine-modified graphene field-effect transistor (PLL@G-FET) for in situ monitoring of extracellular acidosis around cancer cells. PLL is a well-known material with good biocompatibility and is rich in amino groups that are sensitive to hydrogen ions. After a simple drop-casting of PLL on the reduced graphene oxide (RGO) FET surface, the PLL@G-FET was able to realize the real-time monitoring of the localized pH change of cancer cells after the cancer cells were grown on the device. The PLL@G-FET sensor achieved a Nernstian value of 52.9 mV/pH in phosphate buffer saline from pH 4.0 to 8.0. In addition, the sensor exhibited excellent biocompatibility as well as good anti-interference ability in the cell culture medium. Furthermore, the device was used to real-time monitor the extracellular pH changes of MCF-7 cells under the intervention of different concentrations of drugs. This developed pH-sensitive FET provides a new method to study the extracellular acid environment in situ and helps us to enhance our understanding of cancer cell metabolism.
癌细胞的外部酸性环境与正常细胞不同，这对癌症的进展有深远的影响。在这里，我们报告了一种简单的聚赖氨酸修饰的石墨烯场效应晶体管（PLL@G-FET），用于原位监测癌细胞周围的细胞外酸中毒。PLL 是一种具有良好生物相容性的知名材料，并且富含对氢离子敏感的氨基。在将 PLL 简单地滴铸在还原氧化石墨烯（RGO）FET 表面之后，当癌细胞在器件上生长后，PLL@G-FET 能够实时监测癌细胞局部 pH 值的变化。PLL@G-FET 传感器在 pH 值为 4.0 到 8.0 的磷酸盐缓冲盐溶液中实现了 52.9 mV/pH 的 Nernst 值。此外，该传感器在细胞培养液中表现出优异的生物相容性和良好的抗干扰能力。此外，该器件还用于实时监测不同药物浓度干预下 MCF-7 细胞的细胞外 pH 值变化。这种开发的 pH 敏感 FET 为原位研究细胞外酸性环境提供了一种新方法，有助于我们增强对癌细胞代谢的理解。
