College of Medicine, Kyung Hee University, Seoul, Republic of Korea.
Department of Otolaryngology - Head and Neck Surgery, School of Medicine, Kyung Hee University, Seoul, Republic of Korea.
J Int Adv Otol. 2022 Sep;18(5):420-425. doi: 10.5152/iao.2022.21367.
This study evaluated whether recovery from facial paralysis was associated with the expression of Krox-20 and c-Jun genes that are shown to be related to facial nerve regeneration after facial nerve compression and cutting.
The left facial nerves of 24 male Sprague-Dawley rats aged 6 weeks were subjected to crushing or cutting injury. Whisker movements of the vibrissae muscle and blink reflexes of the eyelids were measured on days 4 and 14 after facial nerve injury. The facial nerves on both sides were removed, and the expression of c-Jun and Krox-20 proteins was evaluated by Western blotting.
The level of expression of Krox-20 on day 4 was lower in the crushing group, especially in the cutting group than in the control group (P < .05), but there was no statistically significant difference on day 14 (P > .05). The expression of c-Jun expression was significantly higher in the crushing and cutting groups than in the control group on days 4 and 14 (P < .05).
The degree of facial paralysis was more severe and the recovery rate was lower in the cutting group than in the crushing group. The levels of expression of Krox-20 and c-Jun were associated with facial nerve regeneration after facial nerve injury.
本研究评估了面神经压迫和切断后与面神经再生有关的 Krx-20 和 c-Jun 基因的表达是否与面瘫恢复有关。
24 只 6 周龄雄性 Sprague-Dawley 大鼠的左侧面神经受到挤压或切割损伤。面神经损伤后第 4 天和第 14 天测量触须运动和眼睑眨眼反射。取出两侧面神经，通过 Western blot 评估 c-Jun 和 Krx-20 蛋白的表达。
第 4 天，挤压组，尤其是切割组 Krx-20 的表达水平明显低于对照组（P<0.05），但第 14 天无统计学差异（P>0.05）。第 4 天和第 14 天，挤压组和切割组 c-Jun 的表达均明显高于对照组（P<0.05）。
切割组面瘫程度较严重，恢复率较挤压组低。Krx-20 和 c-Jun 的表达水平与面神经损伤后的面神经再生有关。
