Younis Gamal, Mady Mona, Awad Amal
Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.
Vet World. 2019 Jul;12(7):1078-1084. doi: 10.14202/vetworld.2019.1078-1084. Epub 2019 Jul 21.
The objectives of this study were to investigate the prevalence of in retail chicken meat, ground and processed beef meat, determine their virulence-associated genes, antimicrobial susceptibility pattern, molecular detection of extended-spectrum β-lactamases, and their capability of biofilm formation .
A total of 210 samples (120 retail chicken meat, 30 ground beef, 30 beef burger, and 30 sausage samples) were collected from different retail chicken outlets and markets located at Mansoura city between December 2016 and April 2017. Meat samples were examined bacteriologically for the existence of ; bacterial colonies that displayed positive biochemical properties were subjected to polymerase chain reaction targeting 16 rRNA gene. isolates were tested for their susceptibility to six antimicrobial agents using disk diffusion method. Uniplex PCR was used for screening isolates for the presence of two virulence chromosome-associated genes ( and ), and β-lactamases ( and ). The capability of to form biofilms was detected by tube method.
Thirty isolates (14.29%) were recovered including 19 (15.83%) isolates from chicken meat, 3 (10%) from ground beef, 5 (16.67%) from beef burger, and 3 (10%) from sausage samples. Regarding gene, it was detected in 6.67% (2/30), while gene detected in 20% (6/30) isolates. About 80%, 70%, 63.33%, and 50% of isolates were sensitive to ciprofloxacin, gentamicin, cefotaxime, and streptomycin, respectively, while 83.33% of isolates were resistant to both ampicillin and cephalothin. Interestingly, 21 (70%) isolates had the capability of biofilms formation . Among the multidrug-resistant (MDR) strains, a significant difference (p<0.05) was found between MDR and biofilm formation. However, biofilm formation was correlated with the resistance of the isolates to β-lactam antimicrobials and the presence of β-lactam-resistant genes.
The presence of in chicken meat, ground and processed beef meat represents a significant health risk for meat consumers, which reflects the contamination of slaughterhouses and processing operations, therefore, strict hygienic measures should be applied to minimize carcasses contamination.
本研究的目的是调查零售鸡肉、绞碎牛肉和加工牛肉中[未提及具体细菌名称]的流行情况，确定其毒力相关基因、抗菌药物敏感性模式、超广谱β-内酰胺酶的分子检测以及它们形成生物膜的能力。
2016年12月至2017年4月期间，从曼苏拉市不同的零售鸡肉店和市场共采集了210份样本（120份零售鸡肉、30份绞碎牛肉、30份牛肉汉堡和30份香肠样本）。对肉类样本进行细菌学检查以检测[未提及具体细菌名称]的存在；对显示出阳性生化特性的细菌菌落进行靶向16 rRNA基因的聚合酶链反应。使用纸片扩散法检测[未提及具体细菌名称]分离株对六种抗菌药物的敏感性。采用单重PCR筛选[未提及具体细菌名称]分离株中两个毒力染色体相关基因（[未提及具体基因名称]和[未提及具体基因名称]）以及β-内酰胺酶（[未提及具体酶名称]和[未提及具体酶名称]）的存在情况。通过试管法检测[未提及具体细菌名称]形成生物膜的能力。
共分离出30株[未提及具体细菌名称]分离株（14.29%），其中鸡肉中分离出19株（15.83%），绞碎牛肉中分离出3株（10%），牛肉汉堡中分离出5株（16.67%），香肠样本中分离出3株（10%）。关于[未提及具体基因名称]基因，在6.67%（2/30）的分离株中检测到，而[未提及具体基因名称]基因在20%（6/30）的[未提及具体细菌名称]分离株中检测到。分别约80%、70%、63.33%和50%的[未提及具体细菌名称]分离株对环丙沙星、庆大霉素、头孢噻肟和链霉素敏感，而83.33%的[未提及具体细菌名称]分离株对氨苄西林和头孢菌素均耐药。有趣的是，21株（70%）分离株具有形成生物膜的能力。在多重耐药（MDR）菌株中，MDR与生物膜形成之间存在显著差异（p<0.05）。然而，生物膜形成与分离株对β-内酰胺类抗菌药物的耐药性以及β-内酰胺耐药基因的存在相关。
鸡肉、绞碎牛肉和加工牛肉中[未提及具体细菌名称]的存在对肉类消费者构成了重大健康风险，这反映了屠宰场和加工操作的污染，因此，应采取严格的卫生措施以尽量减少胴体污染。
