Graduate student. Centro Universitário Sagrado Coração - Center for Health Sciences - Bauru (SP), Brazil.
PhD. Universidade do Oeste Paulista - Postgraduate Program in Environment and Regional Development - Presidente Prudente (SP), Brazil.
Acta Cir Bras. 2022 Aug 12;37(5):e370504. doi: 10.1590/acb370504. eCollection 2022.
To study the influence of silver nanoparticles (AgNP) on tissue reaction when incorporated into a polymeric matrix of polyacrylic acid-based (Carbopol®) gel as a proposal for a new low-cost type of biomaterial that is simple to manufacture for use as an antimicrobial and antioxidative dressing.
In-vivo tests of implantation in the subcutaneous tissue of the back of rats were performed using polyethylene tubes in three situations: empty, only the gel, and gel incorporated with AgNP. Then, the tissue reaction was studied by counting inflammatory cells. Additionally, in-vitro tests of the antioxidative and antimicrobial activity of AgNP were performed. The radical 2,2 diphenyl-1 picrylhydrazyl (DPPH) was used to test the antioxidative activity of AgNP using electron spin resonance. The antimicrobial activity of AgNP was determined by minimum inhibitory concentration against the microorganisms: Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli.
The results indicated that AgNP presents antioxidative activity and was able to inhibit the growth of the microorganisms tested. The addition of AgNP in Carbopol® did not alter the tissue inflammatory response (p>0.05, Kruskal-Wallis's test).
The new biomaterial is promising for future use as a dressing for its beneficial properties for regenerative processes.
研究将银纳米粒子（AgNP）掺入聚丙烯酸基（Carbopol®）凝胶的聚合物基质中对组织反应的影响，提出一种新的低成本生物材料，该材料制造简单，可用作具有抗菌和抗氧化功能的敷料。
通过在大鼠背部皮下组织中使用聚乙烯管进行体内试验，共分为三种情况：空管、仅凝胶和凝胶中掺入 AgNP。然后，通过计数炎症细胞来研究组织反应。此外，还进行了 AgNP 的抗氧化和抗菌活性的体外试验。使用电子自旋共振（ESR）技术，使用 2,2-二苯基-1-苦基肼基（DPPH）自由基来测试 AgNP 的抗氧化活性。通过最小抑菌浓度（MIC）测定 AgNP 对金黄色葡萄球菌、铜绿假单胞菌和大肠杆菌等微生物的抗菌活性。
结果表明，AgNP 具有抗氧化活性，能够抑制所测试微生物的生长。在 Carbopol®中添加 AgNP 不会改变组织炎症反应（p>0.05，Kruskal-Wallis 检验）。
这种新的生物材料具有作为再生过程有益的敷料的应用前景。
