Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China.
Joint Laboratory for Deep Blue Fishery Engineering, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, China.
Front Endocrinol (Lausanne). 2022 Aug 1;13:984797. doi: 10.3389/fendo.2022.984797. eCollection 2022.
Spexin (SPX) is an evolutionarily conserved neuropeptide, which was first identified in human proteome by data mining. Two orthologs (SPX1 and SPX2) are present in some non-mammalian species, including teleosts. It has been demonstrated that SPX1 is involved in reproduction and food intake, whereas the functional role of SPX2 is still absent in any vertebrate. The aim of the current study was to evaluate the actions of intraperitoneal injection of endogenous SPX2 peptide on the expression levels of some key reproductive genes of the brain-pituitary axis in half-smooth tongue sole. Our data showed an inhibitory action of SPX2 on brain , , and pituitary , mRNA levels. However, SPX2 had no significant effect on brain , , , , , expression or pituitary expression. On the other hand, SPX2 induced an increase in pituitary expression. Taken together, our results provide initial evidence for the involvement of SPX2 in the regulation of reproduction in vertebrates, which is in accordance with previous studies on SPX1.
Spexin (SPX) 是一种进化上保守的神经肽，最初通过数据挖掘在人类蛋白质组中被发现。两种同源物 (SPX1 和 SPX2) 存在于一些非哺乳动物物种中，包括硬骨鱼。已经证明 SPX1 参与生殖和摄食，而 SPX2 的功能作用在任何脊椎动物中都不存在。本研究的目的是评估内源性 SPX2 肽腹腔注射对半滑舌鳎脑-垂体轴一些关键生殖基因表达水平的影响。我们的数据显示 SPX2 对脑、、和垂体、、mRNA 水平有抑制作用。然而，SPX2 对脑、、、、、表达或垂体 表达没有显著影响。另一方面，SPX2 诱导了垂体 表达的增加。总之，我们的结果为 SPX2 参与脊椎动物生殖调节提供了初步证据，这与之前关于 SPX1 的研究结果一致。
