Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China.
Joint Laboratory for Deep Blue Fishery Engineering, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, China.
Front Endocrinol (Lausanne). 2023 Mar 24;14:1099832. doi: 10.3389/fendo.2023.1099832. eCollection 2023.
LPXRFa, also known as gonadotropin-inhibitory hormone (GnIH), and kisspeptin (Kiss) are two major hypothalamic peptides that modulate the reproductive axis of vertebrates, including teleosts. However, little information is available regarding the actions of nutritional status on the regulation of these two neuroendocrine systems in fish. Herein, we assessed the effects of starvation and refeeding on the expression of , and their receptors ( and respectively) at the brain-pituitary level of half-smooth tongue sole (). Food deprivation for 4 weeks induced a rise in brain as well as brain and pituitary mRNA levels, and refeeding restored brain and expression back to normal. However, pituitary mRNA levels still remained high after 1 week of refeeding. Neither nor transcripts in the pituitary were altered by fasting, but their mRNA levels increased significantly after 1 week of refeeding, and declined back to the control levels after 2 weeks of refeeding. None of brain and along with pituitary transcripts were modified by the nutritional status. In summary, our results revealed an interaction between energy status and the elements of LPXRFa and Kiss systems in the brain-pituitary axis of half-smooth tongue sole. Food deprivation and refeeding differentially regulated the two systems, which provided additional evidence for the involvement of the LPXRFa and Kiss systems in the regulation of reproduction by energy balance in non-mammalian species.
LPXRFa，也被称为促性腺激素抑制激素（GnIH）和 kisspeptin（Kiss），是两种主要的下丘脑肽，调节包括硬骨鱼在内的脊椎动物的生殖轴。然而，关于营养状况对这些两个神经内分泌系统在鱼类中的调节作用的信息知之甚少。在此，我们评估了饥饿和再投喂对半滑舌鳎（）脑垂体水平上 和 及其受体（ 和 分别）表达的影响。4 周的禁食导致脑 以及脑和垂体 mRNA 水平升高，再投喂使脑 和 表达恢复正常。然而，垂体 mRNA 水平在再投喂 1 周后仍保持较高水平。禁食不会改变垂体中的 或 转录本，但再投喂 1 周后其 mRNA 水平显著增加，再投喂 2 周后下降至对照水平。脑 和 以及垂体 转录本均不受营养状态的影响。总之，我们的结果揭示了能量状态与半滑舌鳎脑垂体轴中 LPXRFa 和 Kiss 系统元素之间的相互作用。饥饿和再投喂对这两个系统的调节存在差异，为 LPXRFa 和 Kiss 系统参与非哺乳动物中能量平衡对生殖的调节提供了额外证据。
