声流控单细胞微分配器用于单细胞轨迹控制。

Acoustofluidic cell micro-dispenser for single cell trajectory control.

机构信息

Laboratory for Micro Systems, Department of Mechanical and Aerospace Engineering, Monash University, Clayton, VIC 3800, Australia.

Applied Micro- and Nanotechnology Laboratory, Department of Mechanical and Aerospace Engineering, Monash University, Clayton, VIC 3800, Australia.

出版信息

Lab Chip. 2022 Sep 13;22(18):3533-3544. doi: 10.1039/d2lc00216g.

DOI:10.1039/d2lc00216g

PMID:35979941

Abstract

The precise manipulation of individual cells is a key capability for the study of single cell physiological characteristics or responses to stimuli. Currently, only large cell populations can be transferred with certainty using expensive and laborious flow cytometry platforms. However, when approaching small populations of cells, this task becomes increasingly challenging. Here, we report an effective acoustofluidic micro-dispenser, utilising surface acoustic waves (SAWs), with the ability to trap and release cells on demand, which when combined with an external valve can guide the trajectory of individual cells. We demonstrate single cell trap and release with a single cell trapping effectiveness of 74%, enabling the capability of dispensing a highly controlled amount of cells without any harmful effects. This device has the potential to be easily integrated into a wide range of analytical platforms for applications such as single cell fluorescent imaging and single cell proteomic studies.

摘要

精确操纵单个细胞是研究单细胞生理特征或对刺激反应的关键能力。目前，只有使用昂贵且繁琐的流式细胞术平台才能可靠地转移大量细胞群体。然而，当接近小细胞群体时，这项任务变得越来越具有挑战性。在这里，我们报告了一种有效的声流控微分配器，利用表面声波（SAW），能够按需捕获和释放细胞，当与外部阀结合时，可以引导单个细胞的轨迹。我们展示了单个细胞的捕获和释放，单个细胞的捕获效率为 74%，从而能够以高度可控的方式分配细胞，而不会产生任何有害影响。该设备具有易于集成到各种分析平台的潜力，可用于单细胞荧光成像和单细胞蛋白质组学研究等应用。