Department of Physiology, Keimyung University, School of Medicine, Daegu, Republic of Korea.
Cell Physiology Lab., Department of Physiology, Seoul National University College of Medicine, Seoul, Republic of Korea.
Curr Protoc. 2022 Aug;2(8):e522. doi: 10.1002/cpz1.522.
Distinguishing different contexts is thought to involve a form of pattern separation that minimizes overlap between neural ensembles representing similar experiences. Theoretical models suggest that the dentate gyrus (DG) segregates cortical input patterns before relaying its discriminated output patterns to the CA3 hippocampal field. This suggests that the evaluation of neural ensembles in DG and CA3 could be an important means to investigate the process of pattern separation. In the past, measurement of entorhinal cortex (EC), DG, and CA3 ensembles was largely dependent upon in vivo electrophysiological recording, which is technically difficult. This protocol provides a method to instead measure pattern separation by a molecular method that provides direct spatial resolution at the cellular level. © 2022 Wiley Periodicals LLC. Basic Protocol: Measuring pattern separation by molecular methods.
区分不同的语境被认为涉及一种模式分离形式，这种形式可最大限度地减少代表相似经历的神经集合之间的重叠。理论模型表明，齿状回（DG）在将其区分的输出模式中继到 CA3 海马场之前，会对皮质输入模式进行分离。这表明在 DG 和 CA3 中评估神经集合可能是研究模式分离过程的重要手段。过去，内嗅皮层 (EC)、DG 和 CA3 集合的测量在很大程度上依赖于体内电生理记录，这在技术上具有难度。本方案提供了一种通过分子方法测量模式分离的方法，该方法在细胞水平上提供了直接的空间分辨率。©2022Wiley Periodicals LLC. 基本方案：通过分子方法测量模式分离。
