Department of Biochemistry, Microbiology and Biotechnology, Yerevan State University, Alex Manoogian 1, 0025, Yerevan, Armenia.
The Hamburg Centre for Ultrafast Imaging (CUI), University of Hamburg, Luruper Chaussee 149, 22761, Hamburg, Germany.
Sci Rep. 2022 Aug 18;12(1):14077. doi: 10.1038/s41598-022-17929-y.
In recent years silver nanoparticles (Ag NPs) gained increased and widespread applications in various fields of industry, technology, and medicine. This study describes the green synthesis of silver nanoparticles (Ag NPs) applying a low-molecular-weight fraction (LMF) of Royal Jelly, the nanoparticle characterization, and particularly their antibacterial activity. The optical properties of NPs, characterized by UV-Vis absorption spectroscopy, showed a peak at ~ 430 nm. The hydrodynamic radius and concentration were determined by complementary dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA). The particle morphology was investigated using transmission electron microscopy (TEM), and the crystallinity of the silver was confirmed by X-ray diffraction (XRD). The antibacterial activities were evaluated utilizing Gram-negative and Gram-positive bacteria and colony counting assays. The growth inhibition curve method was applied to obtain information about the corresponding minimum inhibitory concentrations (MIC) and the minimum bactericidal concentrations (MBC) required. Obtained results showed that (i) the sizes of Ag NPs are increasing within the increase of silver ion precursor concentration, (ii) DLS, in agreement with NTA, showed that most particles have dimensions in the range of 50-100 nm; (iii) E. coli was more susceptible to all Ag NP samples compared to B. subtilis.
近年来，纳米银（Ag NPs）在工业、技术和医学等各个领域的应用日益广泛。本研究描述了一种利用蜂王浆低分子质量部分（LMF）来合成纳米银（Ag NPs）的方法，对纳米粒子进行了表征，特别是其抗菌活性。通过紫外可见吸收光谱对 NPs 的光学性质进行了表征，在~430nm 处出现了一个峰值。通过互补动态光散射（DLS）和纳米颗粒跟踪分析（NTA）测定了纳米颗粒的水动力半径和浓度。使用透射电子显微镜（TEM）研究了颗粒形态，并通过 X 射线衍射（XRD）证实了银的结晶度。利用革兰氏阴性菌和革兰氏阳性菌及菌落计数实验评估了抗菌活性。采用生长抑制曲线法获得了相应的最小抑菌浓度（MIC）和最小杀菌浓度（MBC）信息。结果表明：（i）随着银离子前体浓度的增加，Ag NPs 的尺寸增大；（ii）DLS 与 NTA 一致，表明大多数颗粒的尺寸在 50-100nm 范围内；（iii）与枯草芽孢杆菌相比，大肠杆菌对所有 Ag NP 样品更敏感。
