Department of Liver Surgery, Peking Union Medical College (PUMC) Hospital, PUMC & Chinese Academy of Medical Sciences (CAMS), Beijing, China.
Front Immunol. 2022 Aug 2;13:941289. doi: 10.3389/fimmu.2022.941289. eCollection 2022.
The tumor immune microenvironment significantly affects tumor progression, metastasis, and clinical therapy. Its basic cell components include tumor-associated endothelial cells, fibroblasts, and macrophages, all of which constitute the tumor stroma and microvascular network. However, the functions of tumor stromal cells have not yet been fully elucidated. The three-dimensional (3D) model created by 3D bioprinting is an efficient way to illustrate cellular interactions . However, 3D bioprinted model has not been used to explore the effects of stromal cells on cholangiocarcinoma cells. In this study, we fabricated 3D bioprinted models with tumor cells and stromal cells. Compared with cells cultured in two-dimensional (2D) environment, cells in 3D bioprinted models exhibited better proliferation, higher expression of tumor-related genes, and drug resistance. The existence of stromal cells promoted tumor cell activity in 3D models. Our study shows that 3D bioprinting of an immune microenvironment is an effective way to study the effects of stromal cells on cholangiocarcinoma cells.
肿瘤免疫微环境显著影响肿瘤的进展、转移和临床治疗。其基本细胞成分包括肿瘤相关的内皮细胞、成纤维细胞和巨噬细胞，它们共同构成了肿瘤基质和微血管网络。然而，肿瘤基质细胞的功能尚未被充分阐明。三维（3D）生物打印创建的 3D 模型是阐明细胞相互作用的有效方法。然而，3D 生物打印模型尚未用于探索基质细胞对胆管癌细胞的影响。在这项研究中，我们制造了含有肿瘤细胞和基质细胞的 3D 生物打印模型。与在二维（2D）环境中培养的细胞相比，3D 生物打印模型中的细胞增殖更好，肿瘤相关基因的表达更高，且具有耐药性。基质细胞的存在促进了 3D 模型中肿瘤细胞的活性。我们的研究表明，免疫微环境的 3D 生物打印是研究基质细胞对胆管癌细胞影响的有效方法。
