Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, United States.
Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, Washington 98195, United States.
ACS Appl Mater Interfaces. 2022 Aug 31;14(34):38541-38549. doi: 10.1021/acsami.2c10669. Epub 2022 Aug 19.
Poly(dimethylsiloxane) (PDMS) is a commonly used polymer in organ-on-a-chip devices and microphysiological systems. However, due to its hydrophobicity and permeability, it absorbs drug compounds, preventing accurate drug screening applications. Here, we developed an effective and facile method to prevent the absorption of drugs by utilizing a PDMS-PEG block copolymer additive and drug pretreatment. First, we incorporated a PDMS-PEG block copolymer into PDMS to address its inherent hydrophobicity. Next, we addressed the permeability of PDMS by eliminating the concentration gradient via pretreatment of the PDMS with the drug prior to experimentally testing drug absorption. The combined use of a PDMS-PEG block copolymer with drug pretreatment resulted in a mean reduction of drug absorption by 91.6% in the optimal condition. Finally, we demonstrated that the proposed method can be applied to prevent drug absorption in a PDMS-based cardiac microphysiological system, enabling more accurate drug studies.
聚二甲基硅氧烷（PDMS）是器官芯片设备和微生理系统中常用的聚合物。然而，由于其疏水性和渗透性，它会吸收药物化合物，从而无法准确进行药物筛选应用。在这里，我们开发了一种有效且简便的方法，通过使用 PDMS-PEG 嵌段共聚物添加剂和药物预处理来防止药物吸收。首先，我们将 PDMS-PEG 嵌段共聚物掺入 PDMS 中，以解决其固有的疏水性问题。接下来，我们通过在进行药物吸收实验之前用药物对 PDMS 进行预处理来消除浓度梯度，从而解决了 PDMS 的渗透性问题。PDMS-PEG 嵌段共聚物与药物预处理的联合使用导致在最佳条件下药物吸收减少了 91.6%。最后，我们证明了该方法可以应用于防止基于 PDMS 的心脏微生理系统中的药物吸收，从而可以更准确地进行药物研究。
