Department of Burns, Nanfang Hospital, Southern Medical University, Jingxi Street, Baiyun District, Guangdong, 510515, PR China.
Department of Microscopy and Hand and Foot Surgery, Yunfu People's Hospital, Central Laboratory of YunFu People's Hospital, No. 120 Huanshi East Road, Yuncheng District, Yunfu City, 527399, PR China.
J Nanobiotechnology. 2024 Sep 2;22(1):530. doi: 10.1186/s12951-024-02799-5.
Improper management of diabetic wound effusion and disruption of the endogenous electric field can lead to passive healing of damaged tissue, affecting the process of tissue cascade repair. This study developed an extracellular matrix sponge scaffold (KP@Mxene) by incorporating Mxene into an acellular dermal stroma-hydroxypropyl chitosan interpenetrating network structure. This scaffold is designed to couple with the endogenous electric field and promote precise tissue remodelling in diabetic wounds. The fibrous structure of the sponge closely resembles that of a natural extracellular matrix, providing a conducive microenvironment for cells to adhere grow, and exchange oxygen. Additionally, the inclusion of Mxene enhances antibacterial activity(98.89%) and electrical conductivity within the scaffold. Simultaneously, KP@Mxene exhibits excellent water absorption (39 times) and porosity (91%). It actively interacts with the endogenous electric field to guide cell migration and growth on the wound surface upon absorbing wound exudate. In in vivo experiments, the KP@Mxene sponge reduced the inflammatory response in diabetic wounds, increased collagen deposition and arrangement, promoted microvascular regeneration, Facilitate expedited re-epithelialization of wounds, minimize scar formation, and accelerate the healing process of diabetic wounds by 7 days. Therefore, this extracellular matrix sponge scaffold, combined with an endogenous electric field, presents an appealing approach for the comprehensive repair of diabetic wounds.
糖尿病创面渗出液管理不当和内源性电场紊乱可导致损伤组织被动愈合，影响组织级联修复过程。本研究通过将 MXENE 掺入去细胞真皮基质-羟丙基壳聚糖互穿网络结构中，开发了一种细胞外基质海绵支架（KP@Mxene）。该支架旨在与内源性电场偶联，促进糖尿病创面的精确组织重塑。海绵的纤维结构与天然细胞外基质非常相似，为细胞的附着、生长和氧气交换提供了有利的微环境。此外，MXENE 的加入增强了支架的抗菌活性（98.89%）和导电性。同时，KP@Mxene 表现出优异的吸水性（39 倍）和孔隙率（91%）。它在吸收创面渗出液时主动与内源性电场相互作用，引导细胞在创面表面迁移和生长。在体内实验中，KP@Mxene 海绵减轻了糖尿病创面的炎症反应，增加了胶原的沉积和排列，促进了微血管再生，促进了创面的快速再上皮化，最大限度地减少了疤痕形成，并将糖尿病创面的愈合过程加速了 7 天。因此，这种细胞外基质海绵支架与内源性电场相结合，为糖尿病创面的综合修复提供了一种有吸引力的方法。
