Chen Jiun-Yu, Yu Yu-Hsiang
Department of Biotechnology and Animal Science, National Ilan University, Yilan, Taiwan.
J Poult Sci. 2022 Jul 25;59(3):260-271. doi: 10.2141/jpsa.0210139.
The aim of this study was to evaluate the effects of -fermented products (BSFP) on growth performance, intestinal inflammatory gene expression, and cecal microbiota community in broilers challenged with dextran sulfate sodium (DSS) in a 14-day experiment. A total of 32, 1-day-old male broiler chickens (Ross 308), were randomly divided into four groups of eight birds per group and reared individually (=8). The treatments consisted of a control diet without supplementation and DSS challenge, control diet plus 1.5% DSS, control diet plus 1 g/kg BSFP in combination with 1.5% DSS, and control diet plus 3 g/kg of BSFP in combination with 1.5% DSS. The results showed that BSFP supplementation (1 and 3 g/kg) partially improved body weight and average daily gain in broilers under DSS challenge. Relative to DSS treatment alone, BSFP supplementation dose-dependently increased the body weight of broilers at 7 days of age, with the average daily gain being at 1 to 7 days of age. BSFP supplementation (1 and 3 g/kg) alleviated intestinal inflammatory gene expression in broilers under DSS challenge. The richness and evenness of bacterial species in cecal digesta increased in a dose-dependent manner in the groups treated with BSFP (1 and 3 g/kg) in combination with DSS challenge, compared with the control group. Unweighted principal coordinate analysis indicated distinct clusters separating the group treated with 3 g/kg of BSFP in combination with DSS challenge from the other three groups. The abundance of short-chain fatty acid-producing bacteria (genus ) increased and that of mucindegrading bacteria (genus ) decreased in the cecal digesta of broilers fed 3 g/kg of BSFP, compared with the control group. In conclusion, BSFP supplementation dose-dependently improved growth performance, reduced gut inflammation, and regulated the cecal microbiota of broilers exposed to DSS challenge during the starter phase.
本研究的目的是在一项为期14天的实验中，评估β-发酵产品（BSFP）对用硫酸葡聚糖钠（DSS）攻击的肉鸡生长性能、肠道炎症基因表达和盲肠微生物群落的影响。总共32只1日龄雄性肉鸡（罗斯308）被随机分为四组，每组8只，并单独饲养（n = 8）。处理包括不添加且无DSS攻击的对照日粮、对照日粮加1.5%DSS、对照日粮加1 g/kg BSFP并结合1.5%DSS，以及对照日粮加3 g/kg BSFP并结合1.5%DSS。结果表明，添加BSFP（1和3 g/kg）部分改善了DSS攻击下肉鸡的体重和平均日增重。相对于单独的DSS处理，添加BSFP剂量依赖性地增加了7日龄肉鸡的体重，1至7日龄的平均日增重也增加。添加BSFP（1和3 g/kg）减轻了DSS攻击下肉鸡的肠道炎症基因表达。与对照组相比，在添加BSFP（1和3 g/kg）并结合DSS攻击的组中，盲肠内容物中细菌种类的丰富度和均匀度呈剂量依赖性增加。非加权主坐标分析表明，用3 g/kg BSFP结合DSS攻击处理的组与其他三组明显分开。与对照组相比，饲喂3 g/kg BSFP的肉鸡盲肠内容物中产生短链脂肪酸的细菌（属）丰度增加，而粘蛋白降解细菌（属）丰度降低。总之，在起始阶段，添加BSFP剂量依赖性地改善了生长性能，减轻了肠道炎症，并调节了暴露于DSS攻击的肉鸡的盲肠微生物群。
