丁酸梭菌与枯草芽孢杆菌配伍对肉鸡育雏期生长性能、脂质代谢、抗氧化状态及盲肠微生物区系的影响
Effects of compatibility of Clostridium butyricum and Bacillus subtilis on growth performance, lipid metabolism, antioxidant status and cecal microflora of broilers during the starter phase.
作者信息
Zhao Xu, Zhuang Jiarong, Zhang Faling, Li Hongtao, Yu Juan, Wang Chengli, Lv Tengjiao, Li Qingzhen, Zhang Jimei
机构信息
College of Agriculture and Forestry Science, Linyi University, Linyi 276000, China.
Linyi Backbone Biotechnology Co., Ltd., Linyi 276036, China.
出版信息
Anim Biosci. 2024 Nov;37(11):1933-1944. doi: 10.5713/ab.24.0132. Epub 2024 Jun 27.
OBJECTIVE
This study aimed to determine the effects of compatibility of Clostridium butyricum and Bacillus subtilis on growth performance, lipid metabolism, antioxidant status and cecal microflora of broilers during the starter phase.
METHODS
A total of 600 1-day-old Ross 308 broilers were randomly divided into two groups with six replicates in each group. Chickens in the control group were fed a basal diet, while chickens in the experimental group were fed a diet supplemented with 2×108 colony forming units (CFU)/kg of C. butyricum and 1×109 CFU/kg of B. subtilis. The experimental period was 21 days.
RESULTS
Addition of C. butyricum and B. subtilis significantly increased (p<0.05) the body weight and liver nicotinamide adenine dinucleotide phosphate-malic enzyme (NADP-ME) activity of broilers, enhanced (p<0.05) the average daily gain and average daily feed intake of broilers. However, the addition of C. butyricum and B. subtilis did not significantly affect the concentrations of triglyceride and total cholesterol in the serum, the activities of fatty acid synthase and acetyl-CoA carboxylase in the liver, the total antioxidant capacity, glutathione peroxidase activity and malondialdehyde content in the serum and liver. Besides, microbial analysis revealed that supplementation of C. butyricum and B. subtilis increased (p<0.05) the abundance of Firmicutes such as CHKCI001 and Faecalibacterium, decreased (p<0.05) the abundance of Bacteroidota such as Bacteroides and Alistipes. Spearman correlation analysis confirmed that the above cecal microbiota were closely related to the growth performance of broilers (p<0.05). In addition, simultaneous supplementation of C. butyricum and B. subtilis significant affected (p<0.05) 33 different functional pathways such as lipid metabolism and carbohydrate metabolism. This explains the phenomenon of increased growth performance and liver NADP-ME activity in the probiotics group.
CONCLUSION
The compatibility of C. butyricum and B. subtilis could improve the growth of broilers during the starter phase by changing the cecal microflora.
目的
本研究旨在确定丁酸梭菌和枯草芽孢杆菌配伍对雏鸡期肉鸡生长性能、脂质代谢、抗氧化状态及盲肠微生物区系的影响。
方法
将600只1日龄罗斯308肉鸡随机分为两组，每组6个重复。对照组鸡饲喂基础日粮，实验组鸡饲喂添加2×10⁸ 菌落形成单位（CFU）/千克丁酸梭菌和1×10⁹ CFU/千克枯草芽孢杆菌的日粮。试验期为21天。
结果
添加丁酸梭菌和枯草芽孢杆菌显著提高（p<0.05）了肉鸡的体重和肝脏烟酰胺腺嘌呤二核苷酸磷酸苹果酸酶（NADP-ME）活性，增强（p<0.05）了肉鸡的平均日增重和平均日采食量。然而，添加丁酸梭菌和枯草芽孢杆菌对血清中甘油三酯和总胆固醇浓度、肝脏中脂肪酸合酶和乙酰辅酶A羧化酶活性、血清和肝脏中的总抗氧化能力、谷胱甘肽过氧化物酶活性及丙二醛含量无显著影响。此外，微生物分析显示，添加丁酸梭菌和枯草芽孢杆菌增加（p<0.05）了诸如CHKCI001和粪杆菌等厚壁菌门的丰度，降低（p<0.05）了诸如拟杆菌属和艾利斯菌属等拟杆菌门的丰度。Spearman相关性分析证实，上述盲肠微生物群与肉鸡的生长性能密切相关（p<0.05）。此外，同时添加丁酸梭菌和枯草芽孢杆菌显著影响（p<0.05）了33种不同的功能途径，如脂质代谢和碳水化合物代谢。这解释了益生菌组生长性能提高和肝脏NADP-ME活性增加的现象。
结论
丁酸梭菌和枯草芽孢杆菌的配伍可通过改变盲肠微生物区系来改善雏鸡期肉鸡的生长。
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