Ma Xiaojing, Li Hongfei, Zhu Shuntian, Hong Zixuan, Kong Weijing, Yuan Qihang, Wu Runlong, Pan Zihang, Zhang Jing, Chen Yahong, Wang Xi, Wang Kai
Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University, Beijing, China.
Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Beijing, China.
Vasc Biol. 2022 Sep 12;4(1):R44-R57. doi: 10.1530/VB-22-0001. Print 2022 Sep 1.
The emergence of the organoid simulates the native organs and this mini organ offers an excellent platform for probing multicellular interaction, disease modeling and drug discovery. Blood vessels constitute the instructive vascular niche which is indispensable for organ development, function and regeneration. Therefore, it is expected that the introduction of infiltrated blood vessels into the organoid might further pump vitality and credibility into the system. While the field is emerging and growing with new concepts and methodologies, this review aims at presenting various sources of vascular ingredients for constructing vascularized organoids and the paired methodology including de- and recellularization, bioprinting and microfluidics. Representative vascular organoids corresponding to specific tissues are also summarized and discussed to elaborate on the next generation of organoid development.
类器官的出现模拟了天然器官，这种微型器官为探究多细胞相互作用、疾病建模和药物发现提供了一个绝佳的平台。血管构成了具有指导作用的血管生态位，这对于器官发育、功能和再生不可或缺。因此，预计将浸润的血管引入类器官可能会进一步为该系统注入活力和可信度。虽然该领域随着新的概念和方法不断涌现和发展，但本综述旨在介绍构建血管化类器官的各种血管成分来源以及相关方法，包括去细胞化和再细胞化、生物打印和微流体技术。还总结并讨论了与特定组织相对应的代表性血管类器官，以阐述下一代类器官的发展。
