College of Food Science and Engineering, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
College of Food Science and Engineering, Shanxi Agricultural University, Taigu, Shanxi 030801, China; Shanxi Engineering Research Center of Edible Fungi, Taigu, Shanxi 030801, China.
Int J Biol Macromol. 2022 Nov 1;220:659-670. doi: 10.1016/j.ijbiomac.2022.08.117. Epub 2022 Aug 19.
Increasing evidence indicates that type 2 diabetes mellitus (T2DM) is closely related to intestinal bacteria disorders and abnormal hepatic metabolism. Morchella importuna polysaccharide (MIP) shows excellent hypoglycemic activity in vitro. However, the hypoglycemic effect and mechanism of MIP in vivo have yet to be investigated. In this study, the blood glucose, blood lipid and insulin resistance of diabetic mice after MIP intervention were measured to evaluate its hypoglycemic effect. Then, the microbiome and metabolomics were combined to explore the hypoglycemic mechanism of MIP. Results indicated that high dose MIP (400 mg/kg) had significant hypoglycemic effect. Furthermore, MIP could reverse diabetes-induced intestinal disorder by increasing the abundance of Akkermansia, Blautia, Dubosiella, and Lachnospiraceae, as well as decreasing the abundance of Helicobacteraceae. Besides, the hepatic metabolites and complex network systems formed by multiple metabolic pathways were regulated after MIP treatment. Notably, a new biomarker of diabetes (N-P-coumaroyl spermidine) was discovered in this study. Moreover, the significant association between intestinal bacteria and hepatic metabolites was determined by correlations analysis, which in turn confirmed MIP alleviated T2DM via the gut-liver axis. Therefore, these findings elucidated in-depth hypoglycemic mechanisms of MIP and provided a new biomarker for the prevention of diabetes.
越来越多的证据表明，2 型糖尿病（T2DM）与肠道细菌紊乱和异常肝脏代谢密切相关。羊肚菌多糖（MIP）在体外表现出优异的降血糖活性。然而，MIP 在体内的降血糖作用和机制尚未得到研究。在这项研究中，测量了 MIP 干预后糖尿病小鼠的血糖、血脂和胰岛素抵抗，以评估其降血糖作用。然后，将微生物组学和代谢组学相结合，探讨 MIP 的降血糖机制。结果表明，高剂量 MIP（400mg/kg）具有显著的降血糖作用。此外，MIP 可以通过增加 Akkermansia、Blautia、Dubosiella 和 Lachnospiraceae 的丰度，降低 Helicobacteraceae 的丰度，来逆转糖尿病引起的肠道紊乱。此外，MIP 处理后，肝脏代谢物和由多个代谢途径形成的复杂网络系统被调节。值得注意的是，本研究发现了一种糖尿病的新生物标志物（N-对香豆酰腐胺）。此外，通过相关性分析确定了肠道细菌和肝脏代谢物之间的显著关联，这反过来又证实了 MIP 通过肠-肝轴缓解 T2DM。因此，这些发现深入阐明了 MIP 的降血糖机制，并为预防糖尿病提供了一种新的生物标志物。
