School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, China.
Sustainable Functional Biomaterials Laboratory, Department of Wood Science, University of British Columbia.
Acta Biomater. 2022 Oct 1;151:290-303. doi: 10.1016/j.actbio.2022.08.022. Epub 2022 Aug 20.
Zwitterionic polymers have shown promising results in non-fouling and preventing thrombosis. However, the lack of controlled surface coverage hinders their application for biomedical devices. Inspired by the natural biological surfaces, a facile zwitterionic microgel-based coating strategy is developed by the co-deposition of poly (sulfobetaine methacrylate-co-2-aminoethyl methacrylate) microgel (SAM), polydopamine (PDA), and sulfobetaine-modified polyethyleneimine (PES). The SAMs were used to construct controllable morphology by using the PDA combined with PES (PDAS) as the intermediate layer, which can be easily modulated via adjusting the crosslinking degree and contents of SAMs. The obtained SAM/PDAS coatings exhibit high anti-protein adhesive properties and can effectively inhibit the adhesion of cells, bacteria, and platelet through the synergy of high deposition density and controllable morphology. In addition, the stability of SAM/PDAS coating is improved owing to the anchoring effects of PDAS to substrate and SAMs. Importantly, the ex vivo blood circulation test in rabbits suggests that the SAM/PDAS coating can effectively decrease thrombosis without anticoagulants. This study provides a versatile coating method to address the integration of zwitterionic microgel-based coatings with high deposition density and controllable morphology onto various substrates for wide biomedical device applications. STATEMENT OF SIGNIFICANCE: Thrombosis is a major cause of medical device implantation failure, which results in significant morbidity and mortality. In this study, inspired by natural biological surfaces (fish skin and vascular endothelial layer) and the anchoring ability of mussels, we report a convenient and efficient method to firmly anchor zwitterionic microgels using an oxidative co-deposition strategy. The prepared coating has excellent antifouling and antithrombotic properties through the synergistic effect of physical morphology and chemical composition. This biomimetic surface engineering strategy is expected to provide new insights into the clinical problems of blood-contacting devices related to thrombosis.
两性离子聚合物在抗污和防止血栓形成方面表现出了良好的效果。然而，由于缺乏可控的表面覆盖，它们在生物医学设备中的应用受到了限制。受天然生物表面（鱼类皮肤和血管内皮层）和贻贝锚定能力的启发，我们采用一种简便的两性离子微凝胶基氧化共沉积策略，通过聚（磺酸甜菜碱甲基丙烯酸酯-co-2-氨乙基甲基丙烯酸酯）微凝胶（SAM）、聚多巴胺（PDA）和磺酸甜菜碱修饰的聚乙烯亚胺（PES）的共沉积来制备涂层。PDA 与 PES（PDAS）结合作为中间层，可以构建具有可控形态的 SAM，通过调整 SAM 的交联度和含量来轻松调节其形态。所得的 SAM/PDAS 涂层具有高抗蛋白黏附性能，可通过高沉积密度和可控形态的协同作用有效抑制细胞、细菌和血小板的黏附。此外，由于 PDAS 对基底和 SAM 的锚定作用，SAM/PDAS 涂层的稳定性得到了提高。重要的是，在兔子的体外血液循环试验中表明，SAM/PDAS 涂层无需抗凝剂即可有效减少血栓形成。本研究提供了一种通用的涂层方法，可将基于两性离子微凝胶的涂层与高沉积密度和可控形态集成到各种基底上，以满足广泛的生物医学设备应用需求。
