Huang Ningning, Liu Xiangchen, Pei Xiaoqi, Peng Jian, Wei Hongkui
Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China.
Metabolites. 2022 Jul 28;12(8):702. doi: 10.3390/metabo12080702.
Oxylipins (also called eicosanoids) are enzymatically or nonenzymatically generated by oxidation of arachidonic acid (ARA) and are major mediators of ARA effects in the body. Previous studies demonstrated the importance of ARA in infant growth, brain development, immune response, and health. With the developments in lipidomic methodologies, it is important for exploring more ARA-deprived oxylipins to better understand the physiological functions of ARA. The concentrations of oxylipins in feces were determined from days 3 to 21 postnatally of suckling piglets in vivo. Feces were collected at two critical time points of the suckling piglets (3d and 21d after birth) and about 48 oxylipins were analyzed by using a target metabolomics approach based on Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS). Here, 21 oxylipins were derived from ARA, and 11 differential oxylipins (Log2|fold change| ≥ 1.0) at birth 3d and 21d were identified. Particularly, 12-HETE was more abundant in feces at birth 3 days rather than 21 days. Considering that 12-HETE was a racemic mixture of stereoisomers containing the and enantiomers, we further detected the concentrations of 12(S)-HETE and 12(R)-HETE between the two time points by chiral LC-MS/MS analysis. There was no significant difference in the concentrations of 12(S)-HETE and 12(R)-HETE. It was showed that ARA - derived oxylipins might be related to the physiological changes of piglets during growing. Our results provided new information for describing the physiological changes of the piglets over the suckling period.
氧化脂质（也称为类二十烷酸）是由花生四烯酸（ARA）经酶促或非酶促氧化生成的，是ARA在体内发挥作用的主要介质。先前的研究表明ARA在婴儿生长、大脑发育、免疫反应和健康方面具有重要作用。随着脂质组学方法的发展，探索更多ARA衍生的氧化脂质对于更好地理解ARA的生理功能非常重要。在体内测定了哺乳仔猪出生后第3天至第21天粪便中氧化脂质的浓度。在哺乳仔猪的两个关键时间点（出生后3天和21天）收集粪便，并使用基于液相色谱-串联质谱（LC-MS/MS）的靶向代谢组学方法分析了约48种氧化脂质。在此，21种氧化脂质来源于ARA，并鉴定出出生3天和21天时11种差异氧化脂质（Log2|倍数变化|≥1.0）。特别地，12-HETE在出生3天时的粪便中比21天时更为丰富。鉴于12-HETE是一种包含S型和R型对映体的外消旋立体异构体混合物，我们通过手性LC-MS/MS分析进一步检测了两个时间点之间12(S)-HETE和12(R)-HETE的浓度。12(S)-HETE和12(R)-HETE的浓度没有显著差异。结果表明，ARA衍生的氧化脂质可能与仔猪生长过程中的生理变化有关。我们的研究结果为描述仔猪哺乳期的生理变化提供了新的信息。
