College of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China.
Guelph Research and Development Centre, Agriculture and Agri-Food Canada, Guelph, ON N1G 5C9, Canada.
Toxins (Basel). 2022 Aug 5;14(8):537. doi: 10.3390/toxins14080537.
Deoxynivalenol, a mycotoxin that may present in almost all cereal products, can cause huge economic losses in the agriculture industry and seriously endanger food safety and human health. Microbial detoxifications using microbial consortia may provide a safe and effective strategy for DON mitigation. In order to study the interactions involving DON degradation and change in microbial flora, four samples from different natural niches, including a chicken stable (expJ), a sheep stable (expY), a wheat field (expT) and a horse stable (expM) were collected and reacted with purified DON. After being co-incubated at 30 °C with 130 rpm shaking for 96 h, DON was reduced by 74.5%, 43.0%, 46.7%, and 86.0% by expJ, expY, expT, and expM, respectively. After DON (0.8 mL of 100 μg/mL) was co-cultivated with 0.2 mL of the supernatant of each sample (i.e., suspensions of microbial communities) at 30 °C for 96 h, DON was reduced by 98.9%, 99.8%, 79.5%, and 78.9% in expJ, expY, expT, and expM, respectively, and was completely degraded after 8 days by all samples except of expM. DON was confirmed being transformed into de-epoxy DON (DOM-1) by the microbial community of expM. The bacterial flora of the samples was compared through 16S rDNA flux sequencing pre- and post the addition of DON. The results indicated that the diversities of bacterial flora were affected by DON. After DON treatment, the most abundant bacteria belong to (16.1%) and (8.2%) in expJ; (5.9%) and (5.5%) in expY; (13.5%), B1-7BS (13.4%), and RB41 (10.5%) in expT; and (24.1%), (8.8%), and (7.6%) in expM. This first study on the interactions between DON and natural microbial flora provides useful information and a methodology for further development of microbial consortia for mycotoxin detoxifications.
脱氧雪腐镰刀菌烯醇是一种可能存在于几乎所有谷物产品中的真菌毒素，它会给农业产业造成巨大的经济损失，严重威胁食品安全和人类健康。利用微生物群落进行微生物解毒可能为 DON 缓解提供一种安全有效的策略。为了研究涉及 DON 降解和微生物菌群变化的相互作用，从四个不同的自然生态位采集了四个样本，包括鸡舍（expJ）、羊舍（expY）、麦田（expT）和马厩（expM），并与纯化的 DON 进行了反应。在 30°C 下以 130rpm 摇床孵育 96 小时后，expJ、expY、expT 和 expM 分别将 DON 减少了 74.5%、43.0%、46.7%和 86.0%。当将 0.8mL 的 100μg/mL DON 与每个样本的上清液（即微生物群落的悬浮液）0.2mL 在 30°C 下共培养 96 小时后，在 expJ、expY、expT 和 expM 中分别将 DON 减少了 98.9%、99.8%、79.5%和 78.9%，除了 expM 之外，所有样本在 8 天后都将 DON 完全降解。由 expM 的微生物群落将 DON 确认为去环氧 DON（DOM-1）。通过添加 DON 前后的 16S rDNA 通量测序比较了样本的细菌菌群。结果表明，DON 影响了细菌菌群的多样性。在 DON 处理后，最丰富的细菌属于 expJ 中的 （16.1%）和 （8.2%）；expY 中的 （5.9%）和 （5.5%）；expT 中的 （13.5%）、B1-7BS（13.4%）和 RB41（10.5%）；以及 expM 中的 （24.1%）、 （8.8%）和 （7.6%）。这项关于 DON 与天然微生物菌群相互作用的首次研究为进一步开发真菌毒素解毒用微生物群落提供了有用的信息和方法。
