London Research and Development Centre, Agriculture & Agri-Food Canada, 1391 Sandford Street, London, ON N5V 4T3, Canada.
Toxins (Basel). 2022 Aug 9;14(8):544. doi: 10.3390/toxins14080544.
Fumonisin mycotoxins are a family of secondary metabolites produced by and related species, as well as some strains of . Fumonisin contamination of maize is a concern when grown under hot, dry conditions. When present above regulatory levels, there can be effects on animal health. New tools to reduce the toxicity of maize and maize products with high concentrations of fumonisin are needed. Recently, we reported an amine oxidase (AnFAO) from a fumonisin-producing strain capable of oxidatively deaminating intact fumonisins. In this study, AnFAO was used to reduce intact fumonisin concentrations in milled maize flour, whole kernel maize inoculated with fumonisin-producing , and dried distillers' grains with solubles (DDGS). The data showed that milled maize flour incubated with 1 µM AnFAO for 1 h resulted in complete deamination of FB and FB. A greater than 90% reduction in FB concentrations was observed following a simple washing procedure of whole kernel maize in the presence of 1 µM AnFAO for 1 h. Similarly, a ≥86% reduction in FB concentrations was observed in DDGS after 4 h incubation with 1 µM AnFAO. Finally, we engineered the methylotrophic yeast to produce functional AnFAO in both a secreted and intracellular form. These results support the further development and application of AnFAO as a promising tool to remediate fumonisin-contaminated maize and maize products.
伏马菌素真菌毒素是一类由 及相关种属、以及某些 菌株产生的次生代谢物。在炎热干燥的条件下种植玉米时，会出现伏马菌素污染的问题。当存在于监管水平之上时，可能会对动物健康产生影响。需要新的工具来降低含有高浓度伏马菌素的玉米和玉米产品的毒性。最近，我们报道了一种来自产伏马菌素 菌株的胺氧化酶（AnFAO），它能够氧化脱氨完整的伏马菌素。在这项研究中，AnFAO 被用于降低碾磨玉米粉、接种产伏马菌素 的整粒玉米和干燥酒糟（DDGS）中完整伏马菌素的浓度。数据表明，在 1 µM AnFAO 存在下孵育 1 小时的碾磨玉米粉导致 FB 和 FB 完全脱氨。在 1 µM AnFAO 存在下孵育 1 小时，对整粒玉米进行简单的洗涤处理后，观察到 FB 浓度降低了 90%以上。同样，在 1 µM AnFAO 存在下孵育 4 小时后，DDGS 中 FB 浓度降低了≥86%。最后，我们构建了甲基营养酵母 ，使其以分泌和细胞内形式产生功能性 AnFAO。这些结果支持进一步开发和应用 AnFAO 作为一种有前途的工具来修复受伏马菌素污染的玉米和玉米产品。
