Alharbi Mohammad H, Condemine Charlotte, Hesketh Josie, Kayuni Sekeleghe A, Arme Thomas M, Archer John, Jones Sam, LaCourse E James, Makaula Peter, Musaya Janelisa, Stothard J Russell
Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool L3 5QA, UK.
Ministry of Health, Buraydah 52367, Saudi Arabia.
Trop Med Infect Dis. 2023 Feb 18;8(2):126. doi: 10.3390/tropicalmed8020126.
In November 2017, , the key intermediate host for in Africa, was first reported in Lake Malawi, Mangochi District. Two subsequent malacological surveys in 2018 and 2019 confirmed its lacustrine presence, as well as its presence along the Upper Shire River. These surveys provided sufficient specimens for analyses of the genetic structure and a transmission assessment for intestinal schistosomiasis. A total of 76 collected snails were characterized by a DNA sequence analysis of a 650 bp fragment of the mitochondrial cytochrome oxidase subunit 1 (1); by size fractionation of six fluorescently labelled microsatellite loci (Bgμl16, Bgμl, Bpf8, rg6, U-7, and rg9);by denaturing PAGE; and by detection of pre-patent infection by real-time PCR with a TaqMan probe. Five closely related 1 haplotypes were identified, all present within a single location, with only one haplotype common across all the other locations sampled. No allelic size variation was detected with the microsatellites and all loci were monomorphic. Overall, the pre-patent prevalence of spp. was 31%, with infected snails found at several sampling locations. In this part of Lake Malawi, exhibits low genetic diversity and is clearly being exposed to the miracidia of , which is likely facilitating the autochthonous transmission of this parasite.
2017年11月，非洲 病的主要中间宿主首次在曼戈奇区的马拉维湖被发现。随后在2018年和2019年进行的两次贝类学调查证实了它在湖泊中的存在，以及在上谢里河沿岸的存在。这些调查提供了足够的标本用于分析遗传结构和进行肠道血吸虫病的传播评估。通过对线粒体细胞色素氧化酶亚基1的650 bp片段进行DNA序列分析（1）；通过对六个荧光标记微卫星位点（Bgμl16、Bgμl、Bpf8、rg6、U - 7和rg9）进行大小分级；通过变性聚丙烯酰胺凝胶电泳；以及通过使用TaqMan探针的实时PCR检测潜在感染，对总共76只采集的蜗牛进行了特征分析。鉴定出了五个密切相关的1单倍型，均存在于单个位置，在所有其他采样位置仅有一种单倍型是共同的。微卫星未检测到等位基因大小变异，所有位点均为单态。总体而言， 属的潜在感染率为31%，在几个采样位置发现了受感染的蜗牛。在马拉维湖的这一区域， 表现出低遗传多样性，并且显然正在接触 的毛蚴，这可能促进了这种寄生虫的本地传播。
