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小鼠主要尿蛋白（MUP）基因的组织特异性表达：通过cDNA限制性图谱分析和体外翻译对MUP mRNA进行表征。

Tissue-specific expression of major urinary protein (MUP) genes in mice: characterization of MUP mRNAs by restriction mapping of cDNA and by in vitro translation.

作者信息

Shahan K, Derman E

出版信息

Mol Cell Biol. 1984 Nov;4(11):2259-65. doi: 10.1128/mcb.4.11.2259-2265.1984.

DOI:10.1128/mcb.4.11.2259-2265.1984
PMID:6549044
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC369053/
Abstract

The major urinary proteins (MUPs) in mice are coded for by a gene family which consists of ca. 30 members. The number of MUP genes that are expressed is not known. Previous studies have shown that MUP mRNAs are present in several secretory tissues in addition to the liver, in which they were originally identified. In this paper we show, through restriction analysis of MUP cDNAs, that distinct sets of MUP mRNAs are synthesized in each of the tissues studied and that these mRNAs are most likely coded for by different genes. As is shown, MUP mRNAs of different tissues are related to an extent that precludes the use of gene-specific probes in differentiating among them. The regions of homology also include the 3' untranslated regions of MUP mRNAs. The question of differential expression was thus investigated by searching for restriction polymorphisms in MUP mRNAs. We demonstrate that subtle differences in the sequences of even scarce mRNAs can be recognized by this particular approach. In addition, it is shown that MUP mRNAs of different tissues code for different, nonoverlapping sets of polypeptides, as determined by gel electrophoresis of in vitro-translated precursors to MUPs. The relevance of these results to models of evolution of tissue-specific regulation in a multigene family is discussed.

摘要

小鼠的主要尿蛋白（MUPs）由一个约含30个成员的基因家族编码。目前尚不清楚所表达的MUP基因数量。先前的研究表明，除了最初发现MUP mRNA的肝脏外，在其他几个分泌组织中也存在MUP mRNA。在本文中，我们通过对MUP cDNA的限制性分析表明，在所研究的每个组织中都合成了不同的MUP mRNA组，并且这些mRNA很可能由不同的基因编码。如图所示，不同组织的MUP mRNA在一定程度上具有相关性，这使得无法使用基因特异性探针来区分它们。同源区域还包括MUP mRNA的3'非翻译区。因此，通过寻找MUP mRNA中的限制性多态性来研究差异表达问题。我们证明，通过这种特殊方法可以识别出即使是稀少mRNA序列中的细微差异。此外，体外翻译MUP前体的凝胶电泳结果表明，不同组织的MUP mRNA编码不同的、不重叠的多肽组。本文还讨论了这些结果与多基因家族中组织特异性调控进化模型的相关性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9bf/369053/1a95954c007e/molcellb00153-0025-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9bf/369053/bfe8fb9e4461/molcellb00153-0023-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9bf/369053/03192383e83d/molcellb00153-0023-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9bf/369053/48cc22c8f5d5/molcellb00153-0024-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9bf/369053/8c6a890ea6ee/molcellb00153-0024-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9bf/369053/f459b82fea00/molcellb00153-0025-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9bf/369053/14adc93fbb4d/molcellb00153-0025-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9bf/369053/1a95954c007e/molcellb00153-0025-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9bf/369053/bfe8fb9e4461/molcellb00153-0023-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9bf/369053/03192383e83d/molcellb00153-0023-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9bf/369053/48cc22c8f5d5/molcellb00153-0024-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9bf/369053/8c6a890ea6ee/molcellb00153-0024-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9bf/369053/f459b82fea00/molcellb00153-0025-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9bf/369053/14adc93fbb4d/molcellb00153-0025-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9bf/369053/1a95954c007e/molcellb00153-0025-c.jpg

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引用本文的文献

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2
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3

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