Departamento de Medicina Interna, Universidad de La Laguna, Servicio de Medicina Interna, Hospital Universitario de Canarias, Tenerife, Canary Islands, 38320 La Laguna, Spain.
Servicio de Laboratorio, Hospital Universitario de Canarias, Tenerife, Canary Islands, 38320 La Laguna, Spain.
Biomolecules. 2022 Aug 20;12(8):1151. doi: 10.3390/biom12081151.
α-Klotho (Klotho) is an antiaging hormone with anti-inflammatory and antioxidative properties. Some studies suggest that Klotho increases in response to enhanced oxidative damage and inflammation. Alcoholism is a proinflammatory condition. The aim of this study was to analyze the relationship between Klotho and the serum levels of the inflammatory markers in alcoholic liver disease and to assess its prognostic value. We included 184 alcoholics and 35 age- and sex-matched controls. We determined the serum levels of Klotho, the tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-8, and malondialdehyde (MDA), and routine laboratory variables. Patients were followed-up with during 16 ± 18 months; 67 patients died. Klotho levels were higher among cirrhotics (with KW = 37.00 and p < 0.001) and were related to the Child−Pugh score (with KW = 15.96 and p < 0.001) and to the TNF-α (ρ = 0.28; p < 0.001) and MDA (ρ = 0.21; p = 0.006). The child’s groups were associated with mortality, both in the univariate (with the log-rank = 13.56, p = 0.001, Breslow = 12.33, and p = 0.002) and multivariate (with β = 0.43, p = 0.02, and OR = 1.53 (1.07−2.15)) analyses, also introducing Klotho and the TNF-α as dichotomic variables. However, the independent prognostic value of the Child’s groups was displaced by Klotho when only cirrhotics were considered; Klotho, over the median (574.4 pg/mL), was associated with higher mortality (with p = 0.04 and OR = 2.68 (1.06−6.84)). We conclude that Klotho is increased in liver cirrhosis. It is directly related to TNF-α, MDA, and to mortality in cirrhotics.
α-Klotho（Klotho）是一种具有抗炎和抗氧化特性的抗衰老激素。一些研究表明，Klotho 在应对增强的氧化损伤和炎症时会增加。酒精中毒是一种炎症状态。本研究旨在分析 Klotho 与酒精性肝病患者血清炎症标志物水平之间的关系，并评估其预后价值。我们纳入了 184 名酒精中毒患者和 35 名年龄和性别匹配的对照者。我们测定了血清 Klotho、肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6、IL-8 和丙二醛（MDA）以及常规实验室变量。患者随访 16±18 个月，67 例死亡。肝硬化患者 Klotho 水平较高（KW = 37.00，p < 0.001），与 Child-Pugh 评分相关（KW = 15.96，p < 0.001），与 TNF-α（ρ = 0.28；p < 0.001）和 MDA（ρ = 0.21；p = 0.006）相关。在单变量（对数秩检验= 13.56，p = 0.001，Breslow = 12.33，p = 0.002）和多变量（β = 0.43，p = 0.02，OR = 1.53（1.07-2.15））分析中，儿童组与死亡率相关，也将 Klotho 和 TNF-α作为二项变量引入。然而，当仅考虑肝硬化患者时，Child 组的独立预后价值被 Klotho 取代；Klotho 在中位数（574.4 pg/mL）以上与更高的死亡率相关（p = 0.04，OR = 2.68（1.06-6.84））。我们得出结论，Klotho 在肝硬化中增加。它与 TNF-α、MDA 直接相关，与肝硬化患者的死亡率相关。
