Jeon Julie Heejin, Lourenco Jeferson M, Fagan Madison M, Welch Christina B, Sneed Sydney E, Dubrof Stephanie, Duberstein Kylee J, Callaway Todd R, West Franklin D, Park Hea Jin
Department of Nutritional Sciences, College of Family and Consumer Sciences, University of Georgia, Athens, GA 30602, USA.
Department of Animal and Dairy Science, College of Agricultural and Environmental Sciences, University of Georgia, Athens, GA 30602, USA.
Brain Sci. 2022 Aug 21;12(8):1111. doi: 10.3390/brainsci12081111.
Dynamic changes in the oral microbiome have gained attention due to their potential diagnostic role in neurological diseases such as Alzheimer's disease and Parkinson's disease. Traumatic brain injury (TBI) is a leading cause of death and disability in the United States, but no studies have examined the changes in oral microbiome during the acute stage of TBI using a clinically translational pig model. Crossbred piglets (4-5 weeks old, male) underwent either a controlled cortical impact (TBI, = 6) or sham surgery (sham, = 6). The oral microbiome parameters were quantified from the upper and lower gingiva, both buccal mucosa, and floor of the mouth pre-surgery and 1, 3, and 7 days post-surgery (PS) using the 16S rRNA gene. Faith's phylogenetic diversity was significantly lower in the TBI piglets at 7 days PS compared to those of sham, and beta diversity at 1, 3, and 7 days PS was significantly different between TBI and sham piglets. However, no significant changes in the taxonomic composition of the oral microbiome were observed following TBI compared to sham. Further studies are needed to investigate the potential diagnostic role of the oral microbiome during the chronic stage of TBI with a larger number of subjects.
口腔微生物群的动态变化因其在阿尔茨海默病和帕金森病等神经疾病中的潜在诊断作用而受到关注。创伤性脑损伤（TBI）是美国死亡和残疾的主要原因，但尚无研究使用临床转化猪模型研究TBI急性期口腔微生物群的变化。杂种仔猪（4 - 5周龄，雄性）接受了控制性皮质撞击（TBI，n = 6）或假手术（假手术，n = 6）。术前以及术后1、3和7天（PS），使用16S rRNA基因对上下牙龈、双侧颊黏膜和口腔底部的口腔微生物群参数进行定量。与假手术组相比，TBI仔猪在术后7天Faith系统发育多样性显著降低，并且在术后1、3和7天，TBI仔猪和假手术仔猪之间的β多样性存在显著差异。然而，与假手术相比，TBI后未观察到口腔微生物群分类组成的显著变化。需要进一步研究以调查在TBI慢性期口腔微生物群在更多受试者中的潜在诊断作用。
