Eltamany Enas E, Mosalam Esraa M, Mehanna Eman T, Awad Basma M, Mosaad Sarah M, Abdel-Kader Maged S, Ibrahim Amany K, Badr Jihan M, Goda Marwa S
Department of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt.
Department of Biochemistry, Faculty of Pharmacy, Menoufia University, Shebin El-Koum 32511, Egypt.
Biomedicines. 2022 Aug 16;10(8):1986. doi: 10.3390/biomedicines10081986.
L. (Asteraceae) is a wide edible plant that grows in the Mediterranean region. In this study, a phytochemical investigation of L. ethanolic extract led to the isolation of stigmasterol (), ursolic acid (), -amyrin (), azelaic acid (), vanillic acid (), (6S, 7E)-6-hydroxy-4,7-megastigmadien-3,9-dione (S(+)-dehydrovomifoliol) (), 4-hydroxy phenyl acetic acid (), vomifoliol (), ferulic acid (), protocatechuic acid (), kaempferol (), . coumaric acid (), and luteolin (). In addition, the total phenolic content as well as the in vitro antioxidant activity of L. extract were estimated. Moreover, we inspected the potential gonado-protective effect of crude extract, its phenolic fraction, and the isolated coumaric, vanillic, and ferulic acids against methotrexate (MTX)-induced testicular injury in mice. There were seven groups: normal control, MTX control, MTX + crude extract, MTX + phenolic fraction, MTX + isolated coumaric acid, MTX + isolated vanillic acid, and MTX + isolated ferulic acid. MTX was given by i.p. injection of a 20 mg/kg single dose. The crude extract and phenolic fraction were given with a dose of 100 mg/kg/day, whereas the compounds were given at a dose of 10 mg/kg/day. A histopathological examination was done. The testosterone level was detected in serum together with the testicular content of malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD), interleukin 1β (IL-1β), IL-6, tumor necrosis factor alpha (TNF-α), nuclear factor kappa B (NF-κB), B-cell lymphoma 2 (Bcl-2), Bcl-2 associated x protein (Bax), p53, and miR-29a. crude extract, the phenolic fraction, and the isolated compounds showed significant elevation in their levels of testosterone, CAT, SOD, Bcl-2 with a significant decrease in their levels of MDA, TNF-α, IL-1β, IL-6, NF-κB, Bax, P53, and miR-29a compared to those of the MTX control group. In conclusion, mitigated MTX-induced germ cell toxicity via anti-inflammatory, antioxidant, and antiapoptotic effects.
L.（菊科）是一种广泛分布于地中海地区的可食用植物。在本研究中，对L.乙醇提取物进行了植物化学研究，从中分离出了豆甾醇（）、熊果酸（）、α-香树脂醇（）、壬二酸（）、香草酸（）、（6S，7E）-6-羟基-4,7-大柱香波二醇-3,9-二酮（S（+）-脱氢催吐萝芙木醇）（）、4-羟基苯乙酸（）、催吐萝芙木醇（）、阿魏酸（）、原儿茶酸（）、山柰酚（）、香豆酸（）和木犀草素（）。此外，还对L.提取物的总酚含量以及体外抗氧化活性进行了评估。此外，我们还研究了粗提物、其酚类组分以及分离得到的香豆酸、香草酸和阿魏酸对甲氨蝶呤（MTX）诱导的小鼠睾丸损伤的潜在性腺保护作用。实验分为七组：正常对照组、MTX对照组、MTX + 粗提物组、MTX + 酚类组分组、MTX + 分离香豆酸组、MTX + 分离香草酸组和MTX + 分离阿魏酸组。MTX通过腹腔注射20 mg/kg单剂量给药。粗提物和酚类组分的给药剂量为100 mg/kg/天，而化合物的给药剂量为10 mg/kg/天。进行了组织病理学检查。检测了血清中的睾酮水平以及睾丸中丙二醛（MDA）、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、白细胞介素1β（IL-1β）、IL-6、肿瘤坏死因子α（TNF-α）、核因子κB（NF-κB）、B细胞淋巴瘤2（Bcl-2）、Bcl-2相关X蛋白（Bax）、p53和miR-29a的含量。与MTX对照组相比，粗提物、酚类组分和分离得到的化合物的睾酮、CAT、SOD、Bcl-2水平显著升高，而MDA、TNF-α、IL-1β、IL-6、NF-κB、Bax、P53和miR-29a水平显著降低。总之，L.通过抗炎、抗氧化和抗凋亡作用减轻了MTX诱导的生殖细胞毒性。
