Department of Comparative Biomedicine and Food Science, University of Padova, Viale dell'Università 16, 35020 Legnaro-Agripolis, Padova, Italy.
Department of Veterinary Medicine, University of Bari, SP. Casamassima Km.3, 70010 Valenzano, Bari, Italy.
Res Vet Sci. 2021 May;136:97-110. doi: 10.1016/j.rvsc.2021.01.019. Epub 2021 Jan 28.
Skin wound healing may sometimes lead to open sores that persist for long periods and expensive hospitalization is needed. Among different kinds of therapeutic innovative approaches, mesenchymal stem cells (MSCs) and low-temperature atmospheric pressure cold plasma (ionized gas) have been recently tested to improve this regenerative process. To optimize wound healing the present study intended to combine, for the first time, these two novel approaches in a large size animal wound healing model with the aim of assessing the putative dual beneficial effects. Based on clinical, histopathological, and molecular results a synergistic action in a second intention healing wound in sheep has been observed. Experimental wounds treated with cold plasma and MSCs showed a slower but more effective healing compared to the single treatment, as observed in previous studies. The combined treatment improved the correct development of skin appendages and structural proteins of the dermis showing the potential of the dual combination as a safe and effective tool for skin regeneration in the veterinary clinical field.
皮肤创伤愈合有时可能导致长期存在的开放性溃疡，需要昂贵的住院治疗。在不同类型的治疗创新方法中，间充质干细胞 (MSCs) 和低温常压冷等离子体（电离气体）最近已被测试用于改善这种再生过程。为了优化伤口愈合，本研究首次将这两种新方法结合到大型动物伤口愈合模型中，旨在评估潜在的双重有益作用。基于临床、组织病理学和分子结果，观察到绵羊二期愈合伤口的协同作用。与之前的研究相比，用冷等离子体和 MSC 处理的实验性伤口愈合速度较慢，但更有效。联合治疗改善了皮肤附属物和真皮结构蛋白的正确发育，表明这种双重组合作为兽医临床领域皮肤再生的一种安全有效的工具具有潜力。
