从豆荚中分离得到的异黄酮衍生物 Cajanin 的抗炎活性

Natural Product Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheonju 28116, Korea.

Natural Product Central Bank, Korea Research Institute of Bioscience and Biotechnology, Cheonju 28116, Korea.

Int J Mol Sci. 2022 Aug 22;23(16):9492. doi: 10.3390/ijms23169492.

The bioactive components of (Thunb.) DC pods were investigated using bioactivity-guided isolation, and the chemical structures of flavonoids -, isoflavonoid derivatives -, and phenolic compounds and were identified by comparing NMR, MS, and CD spectral data with previously reported spectroscopic data. Compounds - were evaluated for their anti-inflammatory effects on LPS-stimulated RAW264.7 macrophages. Among these compounds, the isoflavonoid derivative cajanin () exhibited the most potent anti-inflammatory activity (IC of NO = 19.38 ± 0.05 µM; IC of IL-6 = 7.78 ± 0.04 µM; IC of TNF-α = 26.82 ± 0.11 µM), exerting its anti-inflammatory effects by suppressing the activation and nuclear translocation of the transcription factor NF-κB by phosphorylating IκB and p65. These results suggested that cajanin () may be a potential candidate for improving the treatment of inflammatory diseases.

采用生物活性导向分离法研究了槐角的生物活性成分，通过与文献报道的光谱数据比较 NMR、MS 和 CD 谱数据，鉴定了黄酮类、异黄酮衍生物类和酚类化合物和。对化合物 - 进行了 LPS 刺激 RAW264.7 巨噬细胞的抗炎作用评价。在这些化合物中，异黄酮衍生物 cajanin () 表现出最强的抗炎活性（NO 的 IC = 19.38 ± 0.05 µM；IL-6 的 IC = 7.78 ± 0.04 µM；TNF-α的 IC = 26.82 ± 0.11 µM），通过磷酸化 IκB 和 p65 抑制转录因子 NF-κB 的激活和核易位来发挥抗炎作用。这些结果表明，cajanin () 可能是改善炎症性疾病治疗的潜在候选药物。