Adl Amini Dominik, Moser Manuel, Chiapparelli Erika, Oezel Lisa, Zhu Jiaqi, Okano Ichiro, Shue Jennifer, Sama Andrew A, Cammisa Frank P, Girardi Federico P, Hughes Alexander P
Spine Care Institute, Hospital for Special Surgery, 535 East 70th Street, New York, NY 10021, USA.
Department of Orthopedic Surgery and Traumatology, Charité University Hospital Berlin, Charitéplatz 1, 10117 Berlin, Germany.
J Clin Med. 2022 Aug 12;11(16):4709. doi: 10.3390/jcm11164709.
Background: Advanced glycation end products (AGEs) have been shown to accumulate in bone and are gaining interest in connective tissue research. Aims: To investigate the intrarater reliability, two-timepoint agreement and correlations within and between two commercially available skin autofluorescence (SAF) AGE devices. Methods: Healthy volunteers were enrolled in a prospective study at a single academic institution. Each participant underwent SAF analysis by two different, commercially available devices on two occasions, 14 days apart. Upon enrollment, a general survey about the participant’s lifestyle and health status was completed and followed up on for any changes at timepoint two. Results: In total, 40 participants (F:M ratio 5:3) with an average age of 39.0 ± 12.5 years were analyzed. For the AGE reader (skin) and AGE sensor (fingertip), both intrarater reliability and two-timepoint agreement were excellent with an interclass correlation coefficient (ICC) > 0.90 and a strong correlation within both machines. However, there was no correlation between both machines for either timepoint. In total, 4 participants were identified as outliers above the +2SD. Additionally, 5 participants with dark-colored skin could not be measured with the AGE reader at timepoint one and 4 at timepoint two. In contrast, all participants were able to undergo SAF analysis with the AGE sensor, irrespective of their skin type. Conclusions: Both machines showed excellent intrarater reliability and two-timepoint agreement, but the skin AGE reader might have limited applicability in individuals with dark-colored skin. Future research on AGEs might take our findings into consideration.
晚期糖基化终末产物（AGEs）已被证明会在骨骼中蓄积，并且在结缔组织研究中越来越受到关注。目的：研究两种市售皮肤自发荧光（SAF）AGE检测设备内部以及之间的评分者内信度、两个时间点的一致性及相关性。方法：在单一学术机构对健康志愿者进行一项前瞻性研究。每位参与者在两个不同的时间点，间隔14天，使用两种不同的市售设备进行SAF分析。入组时，完成关于参与者生活方式和健康状况的一般调查，并在时间点二对任何变化进行随访。结果：共分析了40名参与者（女性与男性比例为5:3），平均年龄为39.0±12.5岁。对于AGE阅读器（皮肤）和AGE传感器（指尖），评分者内信度和两个时间点的一致性均极佳，组内相关系数（ICC）>0.90，且两台机器内部均有很强的相关性。然而，两个时间点两台机器之间均无相关性。总共识别出4名高于+2SD的异常值参与者。此外，5名皮肤颜色较深的参与者在时间点一无法用AGE阅读器测量，4名在时间点二无法测量。相比之下，所有参与者均能够使用AGE传感器进行SAF分析，无论其皮肤类型如何。结论：两台机器均显示出极佳的评分者内信度和两个时间点的一致性，但皮肤AGE阅读器在皮肤颜色较深的个体中适用性可能有限。未来关于AGEs的研究可能需要考虑我们的发现。
